ALTERED SARCOPLASMIC-RETICULUM FUNCTION AFTER HIGH-INTENSITY EXERCISE
ALTERED SARCOPLASMIC-RETICULUM FUNCTION AFTER HIGH-INTENSITY EXERCISE
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DOI：
10.1152/jappl.1989.67.5.2072
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发表时间：
1989-11-01
影响因子：
3.3
通讯作者：
GOLLNICK, PD
中科院分区：
文献类型：
--
作者：
BYRD, SK;MCCUTCHEON, LJ;GOLLNICK, PD
本研究探讨了急性高强度运动对肌浆网Ca 2+摄取速率和能力以及Ca 2+刺激的腺苷三磷酸酶（ATP酶）活性的影响以及这些影响的可逆性。纯种马在高速跑步机上以最大O2摄取量跑步，直到疲劳。肌肉温度和活检样品收集在休息，运动后立即，30和60分钟后运动。分别在安静时和运动后5 min采集血样。肌肉和血液（乳酸盐浓度）比运动前的值高出三倍和五倍。运动后即刻的肌肉温度和pH值分别为43 ℃和6.55，但在运动后60分钟接近静止值。运动后即刻，肌肉匀浆和肌浆网的初始钙摄取速率和最大钙摄取能力显著降低。Ca ~（2+）刺激的ATP酶活性降低可抑制这种抑制作用。然而，钙摄取（速率和容量）和Ca 2 +-ATP酶活性已恢复到正常的运动后60分钟。这些研究结果表明，高强度运动后肌浆网功能的变化可能是由肌肉pH值和/或温度的局部变化引起的，但不是持续的。
This study examined the effects of acute high-intensity exercise on the rate and capacity of Ca2+ uptake and Ca2+-stimulated adenosinetriphosphatase (ATPase) activity of the sarcoplasmic reticulum and the reversibility of these effects. Thoroughbred horses were run at maximal O2 uptake on a high-speed treadmill until fatigued. Muscle temperatures and biopsy samples were collected at rest, immediately after exercise, and 30 and 60 min after exercise. Blood samples were collected at rest and 5 min after exercise. Muscle and blood (lactate concentration) were three- and fivefold greater than pre-exercise values. Muscle temperature and pH immediately after post-exercise were 43.degree.C and 6.55, respectively, but approached rest values by 60 min after exercise. The initial rate and maximal capacity of Ca2+ uptake of muscle homogenates and isolated sarcoplasmic reticulum were significantly depressed immediately after exercise. This depression was paralleled by decreased activity of the Ca2+-stimulated ATPase. However, both Ca2+ uptake (rate and capacity) and Ca2+-ATPase activity had returned to normal by 60 min after exercise. These findings demonstrate that changes in sarcoplasmic reticulum function after high-intensity exercise may be induced but not sustained by local changes in muscle pH and/or temperature.