Root proteomics reveals cucumber 24-epibrassinolide responses under Ca(NO3)2 stress
Root proteomics reveals cucumber 24-epibrassinolide responses under Ca(NO3)2 stress
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根蛋白质组学揭示了 Ca(NO3)(2) 胁迫下黄瓜 24-表油菜素内酯的反应
DOI：
10.1007/s00299-016-1940-z
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发表时间：
2016-05-01
影响因子：
6.2
通讯作者：
Guo, Shirong
中科院分区：
文献类型：
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作者：
An, Yahong;Zhou, Heng;Guo, Shirong
NO3-和Ca 2+是设施栽培土壤次生盐渍化的主要阴、阳离子。植物甾醇类化合物（BRs）是一类甾体植物激素，具有调节多种重要的生理和发育过程的作用，可用于抵御非生物胁迫。采用双向电泳结合MALDI-TOF/TOF MS技术，研究了外源24-表油菜素（EBL）对Ca（NO3）2胁迫下黄瓜幼苗根系蛋白质组变化的影响。共80个差异积累的蛋白质点响应于应力和/或外源EBL被确定和分组到不同类别的生物过程根据基因本体论。Ca（NO3）2胁迫诱导了与氮代谢和木质素合成相关的蛋白质的表达，而抑制了与细胞骨架组织和细胞壁中性糖代谢相关的蛋白质的表达。然而，丰富的蛋白质积累参与蛋白质修饰和降解、抗氧化和解毒的防御机制以及外源EBL合成木质素可能在耐盐性中发挥重要作用。进行实时定量PCR以研究BR信号传导。Ca（NO3）2胁迫诱导BR信号转导。外源EBL能缓解Ca（NO3）2胁迫下黄瓜根系的各项指标，有效降低Ca 2+含量，提高K+含量。本研究揭示了Ca（NO3）2胁迫下EBL诱导黄瓜幼苗根系差异表达的蛋白质和BR信号相关的mRNA，为进一步了解EBL诱导黄瓜幼苗的抗盐性奠定了基础。缓解机制为提高其他园艺植物对Ca（NO3）（2）胁迫的耐受性提供了有价值的见解。
NO3- and Ca2+ are the main anion and cation of soil secondary salinization during greenhouse cultivation. Brassinosteroids (BRs), steroidal phytohormones, regulate various important physiological and developmental processes and are used against abiotic stress. A two-dimensional electrophoresis gel coupled with MALDI-TOF/TOF MS was performed to investigate the effects of exogenous 24-epibrassinolide (EBL) on proteomic changes in cucumber seedling roots under Ca(NO3)(2) stress. A total of 80 differentially accumulated protein spots in response to stress and/or exogenous EBL were identified and grouped into different categories of biological processes according to Gene Ontology. Under Ca(NO3)(2) stress, proteins related to nitrogen metabolism and lignin biosynthesis were induced, while those related to cytoskeleton organization and cell-wall neutral sugar metabolism were inhibited. However, the accumulation of abundant proteins involved in protein modification and degradation, defence mechanisms against antioxidation and detoxification and lignin biosynthesis by exogenous EBL might play important roles in salt tolerance. Real-time quantitative PCR was performed to investigate BR signalling. BR signalling was induced intracellularly under Ca(NO3)(2) stress. Exogenous EBL can alleviate the root indices, effectively reduce the Ca2+ content and increase the K+ content in cucumber roots under Ca(NO3)(2) stress. This study revealed the differentially expressed proteins and BR signalling-associated mRNAs induced by EBL in cucumber seedling roots under Ca(NO3)(2) stress, providing a better understanding of EBL-induced salt resistance in cucumber seedlings. The mechanism for alleviation provides valuable insight into improving Ca(NO3)(2) stress tolerance of other horticultural plants.