Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity
Phototrophic purple bacteria as optoacoustic in vivo reporters of macrophage activity
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DOI：
10.1038/s41467-019-09081-5
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发表时间：
2019-03-13
影响因子：
16.6
通讯作者：
Stiel，Andre C.
中科院分区：
文献类型：
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作者：
Peters，Lena;Weidenfeld，Ina;Stiel，Andre C.
实体瘤的形态学、生理学和免疫学表现出空间异质性，这使我们对癌症进展和治疗反应的理解复杂化。了解空间异质性需要解剖和病理生理肿瘤信息的高分辨率体内成像。我们介绍了Rhododophylla作为细菌报告的多光谱光声（光声）层析（MSOT）。我们发现，内源性bacteriochlorophyllainRhodopyrine产生了强烈的光声信号>800 nm远离干扰内源性吸收剂。重要的是，我们的研究结果表明，依赖于肿瘤内巨噬细胞活性的Rhodododiasis光谱特征的变化可用于揭示肿瘤微环境的异质性。在纵向研究中采用非侵入性高分辨率MSOT，我们显示了荷4T1和CT26.WT肿瘤模型小鼠中Rhodocyte光谱分布的时空变化。Rhododopsis的遗传修饰，从而感官和治疗功能的可访问性表明治疗诊断平台生物的潜力。
Τhe morphology, physiology and immunology, of solid tumors exhibit spatial heterogeneity which complicates our understanding of cancer progression and therapy response. Understanding spatial heterogeneity necessitates high resolution in vivo imaging of anatomical and pathophysiological tumor information. We introduceRhodobacteras bacterial reporter for multispectral optoacoustic (photoacoustic) tomography (MSOT). We show that endogenous bacteriochlorophyllainRhodobactergives rise to strong optoacoustic signals >800 nm away from interfering endogenous absorbers. Importantly, our results suggest that changes in the spectral signature ofRhodobacterwhich depend on macrophage activity inside the tumor can be used to reveal heterogeneity of the tumor microenvironment. Employing non-invasive high resolution MSOT in longitudinal studies we show spatiotemporal changes ofRhodobacterspectral profiles in mice bearing 4T1 and CT26.WT tumor models. Accessibility ofRhodobacterto genetic modification and thus to sensory and therapeutic functions suggests potential for a theranostic platform organism.