THE EXPRESSION OF A RAB-RELATED GENE, RAB18, IS INDUCED BY ABSCISIC-ACID DURING THE COLD-ACCLIMATION PROCESS OF ARABIDOPSIS-THALIANA (L) HEYNH
THE EXPRESSION OF A RAB-RELATED GENE, RAB18, IS INDUCED BY ABSCISIC-ACID DURING THE COLD-ACCLIMATION PROCESS OF ARABIDOPSIS-THALIANA (L) HEYNH
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DOI：
10.1007/bf00027165
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发表时间：
1992-12-01
影响因子：
5.1
通讯作者：
PALVA, ET
中科院分区：
文献类型：
--
作者：
LANG, V;PALVA, ET
我们已经从拟南芥中分离到一个与Rab相关的(对ABA有反应的)基因Rab18。该基因编码一个亲水性的富含甘氨酸的蛋白(18.5 kDa)，它含有其他植物中类似RAB蛋白所特有的保守的富含丝氨酸和赖氨酸的结构域。在低温、水分胁迫或外源ABA胁迫下，Rab18基因在植物体内积累，而在热激条件下不表达。在ABA合成突变体ABA-1、ABA反应突变体ABI-1或用类胡萝卜素合成抑制剂Furidone处理的野生型植物中，与胁迫相关的Rab18 mRNA的积累显著减少。外源ABA处理可以诱导ABA-1突变体中Rab18基因的表达，但不能诱导ABI-1突变体中Rab18基因的表达。这些结果为拟南芥Rab18基因的ABA依赖性调控提供了直接的遗传学证据。
We have isolated a rab-related (responsive to ABA) gene, rab18 from Arabidopsis thaliana. The gene encodes a hydrophilic, glycine-rich protein (18.5 kDa), which contains the conserved serine- and lysine-rich domains characteristic of similar RAB proteins in other plant species. The rab18 mRNA accumulates in plants exposed to low temperature, water stress or exogenous ABA but not in plants subjected to heat shock. This stress-related accumulation of the rab18 mRNA is markedly decreased in the ABA-synthesis mutant aba-1, the ABA-response mutant abi-1 or in wild-type plants treated with the carotenoid synthesis inhibitor, fluridone. Exogenous ABA treatment can induce the rab18 mRNA in the aba-1 mutant but not in the abi-1 mutant. These results provide direct genetic evidence for the ABA-dependent regulation of the rab18 gene in A. thaliana.