Berberine Regulates Treg/Th17 Balance to Treat Ulcerative Colitis Through Modulating the Gut Microbiota in the Colon.
Berberine Regulates Treg/Th17 Balance to Treat Ulcerative Colitis Through Modulating the Gut Microbiota in the Colon.
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小檗碱通过调节结肠肠道微生物群调节 Treg/Th17 平衡来治疗溃疡性结肠炎
DOI：
10.3389/fphar.2018.00571
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发表时间：
2018
影响因子：
5.6
通讯作者：
Zhuang P
中科院分区：
文献类型：
--
作者：
Cui H;Cai Y;Wang L;Jia B;Li J;Zhao S;Chu X;Lin J;Zhang X;Bian Y;Zhuang P
小檗碱（BBR）是从中药大黄、黄柏和小檗中分离得到的一种生物碱，广泛用于治疗溃疡性结肠炎（UC）。然而，BBR治疗UC的机制尚不清楚。在这项研究中，我们研究了调节性T细胞（Treg）和辅助性T细胞17（Th 17）在葡聚糖硫酸钠（DSS）诱导的UC小鼠模型中的活性，在BBR给药后。我们还使用16 S rRNA分析研究了肠道微生物群组成的变化。我们还研究了BBR是否可以通过改变肠道微生物群来调节Treg/Th 17平衡。通过抗生素治疗和粪便移植的组合消耗肠道微生物群进一步证实了该机制。结果显示，BBR治疗可以改善DSS诱导的UC模型中Treg/Th 17平衡。BBR还减少了肠道微生物群的多样性，并干扰了脱硫弧菌、真杆菌和拟杆菌的相对丰度。此外，BBR处理在肠道微生物群耗尽后不影响Treg/Th 17平衡。我们的研究结果还表明，粪便移植从BBR治疗的小鼠可以减轻UC和调节Treg/Th 17平衡。总之，我们的研究提供了BBR通过改变肠道微生物群和调节Treg/Th 17平衡来预防UC的证据。
Berberine (BBR), an alkaloid isolated from Rhizoma Coptidis, Cortex Phellode, and Berberis, has been widely used in the treatment of ulcerative colitis (UC). However, the mechanism of BBR on UC is unknown. In this study, we investigated the activities of T regulatory cell (Treg) and T helper 17 cell (Th17) in a dextran sulfate sodium (DSS)-induced UC mouse model after BBR administration. We also investigated the changes of gut microbiota composition using 16S rRNA analysis. We also examined whether BBR could regulate the Treg/Th17 balance by modifying gut microbiota. The mechanism was further confirmed by depleting gut microbiota through a combination of antibiotic treatment and fecal transplantations. Results showed that BBR treatment could improve the Treg/Th17 balance in the DSS-induced UC model. BBR also reduced diversity of the gut microbiota and interfered with the relative abundance of Desulfovibrio, Eubacterium, and Bacteroides. Moreover, BBR treatment did not influence the Treg/Th17 balance after the depletion of gut microbiota. Our results also revealed that fecal transplantation from BBR-treated mice could relieve UC and regulate the Treg/Th17 balance. In conclusion, our study provides evidence that BBR prevents UC by modifying gut microbiota and regulating the balance of Treg/Th17.