The two classes of genes for the major potato tuber protein, patatin, are differentially expressed in tubers and roots.
The two classes of genes for the major potato tuber protein, patatin, are differentially expressed in tubers and roots.
复制标题
马铃薯主要块茎蛋白 patatin 的两类基因在块茎和根中的表达存在差异。
DOI：
10.1093/nar/15.5.1979
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发表时间：
1987
影响因子：
14.9
通讯作者：
Park,WD
中科院分区：
文献类型：
--
作者：
Pikaard,CS;Brusca,JS;Hannapel,DJ;Park,WD
马铃薯主要块茎蛋白Patatin是一个40kd的糖蛋白家族，占块茎中可溶蛋白的40%，但在其他组织中通常检测不到。用熔融火箭免疫电泳法检测了根中不同于块茎中的巴曲丁。SDS-聚丙烯酰胺凝胶的Western blotting显示，根部的Patatin具有与块茎Patatin isofonns不同的分子量分布，但体外翻译产物的免疫沉淀表明，两种组织中的Patatin前体的分子量相似。这表明，块茎和根的翻译后加工可能不同。Northern杂交显示，块茎和根部Patatin的mRNAs大小相似，但块茎转录产物的含量约为前者的100倍。5‘SI核苷酸和引物延伸图谱表明，在根中表达的Patatin转录本(II类转录本)是在块茎中表达的Patatin转录子的子集(I类和II类)。第II类Patatin mRNAs不同于第I类转录本，因为在起始密码子的上游插入了22个核苷酸。这些数据表明，Patatin多基因家族在块茎和根中的表达存在差异调节。
Tfie major potato tuber protein, patatin, is a family of 40kd glycoproteins that constitutes forty per cent of the soluble protein in tubers but is generally undetectable in other tissues. Fused rocket immunoelectro-phoresis was used to detect in roots patatin that is imnunologically different from tuber patatin. Western blots of SDS-polyacrylamide gels show root patatin to have a different molecular weight distribution than tuber patatin isofonns, but immunoprecipitation of in vitro translation products shows the patatin precursors to be of similar molecular weight in both tissues. This suggests that post-translational processing may differ in tubers and roots. Northern blots show that tuber and root patatin mRNAs are of similar size, but tuber transcripts are about 100-fold more abundant. 5′ SI nucleaso and primer extension mapping suggests the class of patatin transcripts expressed in roots ( class II transcripts) to be a subset of patatin transcripts expressed in tubers (classes I and II). Class II patatin mRNAs differ from class I transcripts by the presence of a 22 nucleotide insertion just upstream of the initiation codon. These data demonstrate that expression of the patatin multigene family is differentially regulated in tubers and roots.