Recognition by xenogeneic cytotoxic T lymphocytes of cells expressing HLA-A2 or HLA-B7 after DNA-mediated gene transfer.
Recognition by xenogeneic cytotoxic T lymphocytes of cells expressing HLA-A2 or HLA-B7 after DNA-mediated gene transfer.
复制标题
DNA 介导的基因转移后，异种细胞毒性 T 淋巴细胞对表达 HLA-A2 或 HLA-B7 的细胞进行识别。
DOI：
10.1073/pnas.80.16.5056
复制
发表时间：
1983
影响因子：
11.1
通讯作者：
Engelhard,VH
中科院分区：
文献类型：
--
作者：
Herman,A;Parham,P;Weissman,SM;Engelhard,VH
表达HLA-A2或-B7抗原的小鼠L细胞在用单纯疱疹病毒胸苷激酶基因和含有HLA-A2或-B7基因的基因组克隆共转化胸苷激酶阴性细胞后分离。单克隆抗体结合分析表明，这些细胞的HLA抗原的稳定细胞表面表达水平高达人B类淋巴母细胞系JY表达量的40%。由L细胞表达的HLA-A2和-B7抗原保留了所有抗体定义的重链相关抗原决定簇，但缺乏与人β 2-微球蛋白相关的决定簇。这些HLA转化体能够作为单克隆细胞毒性T淋巴细胞（CTL）的靶标，所述CTL特异性识别由JY细胞表达的HLA-B7或-A2抗原。然而，相对于JY细胞系，单个CTL的裂解效率为50-99%。此外，并非所有这些CTL都能够裂解适当的转化体。由于抗原出现的血清学标准是结构完整的，并在高水平表达，这些结果表明，HLA重链与小鼠，而不是人，β 2-微球蛋白的互补可能会改变抗原决定簇，是重要的CTL识别。
Murine L cells expressing HLA-A2 or -B7 antigens were isolated after cotransformation of thymidine kinase-negative cells with the herpes simplex virus thymidine kinase gene and the genomic clones containing either the HLA-A2 or -B7 genes. Monoclonal antibody binding analyses demonstrated the stable cell surface expression of HLA antigens by these cells at levels of up to 40% of the amount expressed by the human B lymphoblastoid cell line, JY. The HLA-A2 and -B7 antigens expressed by the L cells retained all of the antibody-defined, heavy-chain-associated antigenic determinants but lacked those determinants associated with human beta 2-microglobulin. These HLA transformants were capable of functioning as targets for monoclonal cytotoxic T lymphocytes (CTL) that specifically recognize the HLA-B7 or -A2 antigens expressed by JY cells. However, the efficiency of lysis, relative to the JY cell line, was 50-99% for individual CTL. In addition, not all of these CTL were capable of lysing the appropriate transformants. Because the antigens appear by serological criteria to be structurally intact and expressed at high levels, these results suggest that the complementation of the HLA heavy chains with mouse, rather than human, beta 2-microglobulin may alter the antigenic determinants that are important for CTL recognition.