Biomimetic approach for root caries prevention using a proanthocyanidin-rich agent.
Biomimetic approach for root caries prevention using a proanthocyanidin-rich agent.
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使用富含原花青素的试剂预防根部龋齿的仿生方法。
DOI：
10.1159/000330599
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发表时间：
2011
期刊：
影响因子：
4.2
通讯作者：
Bedran-Russo,AK
中科院分区：
文献类型：
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作者：
Pavan,S;Xie,Q;Hara,AT;Bedran-Russo,AK
本研究的目的是评价富含原花青素的葡萄籽提取物（GSE）对离体牙根牙本质脱矿的影响。根碎片从健全的人类牙齿获得。将片段随机分配到不同的处理溶液中：GSE、氟化物（F）、GSE+ F和蒸馏水（对照）。每天处理样品30分钟，并使用脱矿循环进行pH循环人工龋方案（2.2 mM CaCl 2× H2O，2.2 mM KH 2 PO 4，50 mM乙酸，pH 4.3）处理6 h，然后进行再矿化循环（20 mM HEPES，2.25 mM CaCl 2× H 2 O，1.35 mM KH 2 PO 4，130 mM KCl，pH 7.0）孵育17.5 h。在处理/pH循环18天后，通过横向显微放射照相术测定矿物质损失（ΔZ）和损伤深度（LD）。与对照组相比，GSE能够最小化ΔZ和LD（p< 0.0001）。GSE+ F组和F组的ΔZ和LD值最低（p< 0.05），两者之间无统计学显著差异（分别为p= 0.554和p= 0.726）。使用GSE强化根牙本质的仿生方法导致根脱矿率降低，并且可以与F结合使用以预防根龋。
The aim of this study was to evaluate the effects of a proanthocyanidin-rich grape seed extract (GSE) on the in vitro demineralization of root dentine. Root fragments were obtained from sound human teeth. The fragments were randomly assigned to different treatments solutions: GSE, fluoride (F), GSE+ F and distilled water (control). Samples were treated daily for 30 min and subjected to a pH cycling artificial caries protocol using demineralization cycles (2.2 mM CaCl 2× H 2 O, 2.2 mM KH 2 PO 4, 50 mM acetic acid, pH 4.3) for 6 h and remineralization cycles (20 mM HEPES, 2.25 mM CaCl 2× H 2 O, 1.35 mM KH 2 PO 4, 130 mM KCl, pH 7.0) for 17.5 h. Mineral loss (ΔZ) and lesion depth (LD) were determined after 18 days of treatment/pH cycling, by transverse microradiography. GSE was able to minimize ΔZ and LD compared with the control group (p< 0.0001). The GSE+ F and F groups showed the lowest values of ΔZ and LD (p< 0.05), with no statistically significant differences between them (p= 0.554 and p= 0.726, respectively). A biomimetic approach to strengthen root dentine using GSE results in decreased rates of root demineralization and may be used in conjunction with F to prevent root caries.