PRECIPITATING AUTO-ANTIBODIES IN CONNECTIVE TISSUE DISEASES
PRECIPITATING AUTO-ANTIBODIES IN CONNECTIVE TISSUE DISEASES
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DOI：
10.1136/ard.21.4.360
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发表时间：
1962-01-01
影响因子：
27.4
通讯作者：
MCELHINNEY, AJ
中科院分区：
文献类型：
--
作者：
ANDERSON, JR;BUCHANAN, WW;MCELHINNEY, AJ
通过琼脂扩散技术检查 188 名结缔组织疾病患者和 353 名“对照”患者的血清样本中是否存在与各种人体组织的盐水提取物反应产生沉淀的因子。在 57 份测试血清样本和 8 份对照血清样本中观察到沉淀。阳性标本的沉淀滴度最高为1分之512。对阳性标本的对比试验表明，其中存在多种诱发因素。对其中的四种进行了进一步研究，并提出证据表明它们是与正常细胞成分发生反应的自身抗体。四种相关细胞成分之一被认为是脱氧核糖核酸 (DNA)；其他三人身份仍然不明。四种抗体的发病率提供了各种类型结缔组织疾病之间的进一步联系。同时，个体结缔组织疾病往往与特定抗体相关。在患有“副球蛋白血症”、慢性甲状腺炎、肝病、再生障碍性贫血和结节病的“对照”患者的血清中检测到促发因素。将沉淀素测试的结果与工作人员使用 Gadjusek 的“自身免疫补体固定反应”(AlCF) 描述的结果进行比较，并建议对后一种技术报告的阴性自体测试进行解释。
Specimens of serum from 188 patients with connective tissue diseases and from 353 "control" patients were examined by an agar-diffusion technique for the presence of factors reacting to give precipitation with saline extracts of various human tissues. Precipitation was observed with 57 of the test serum specimens and with eight of the controls. The precipitating titers of positive specimens ranged up to 1 in 512. Comparative tests upon the positive specimens showed the occurrence among them of several precipitating factors. Four of these were investigated further, and evidence is presented that they are autoantibodies which react with normal cellular constituents. One of the four cellular constituents concerned is considered to be deoxyribonucleic acid (DNA); the other three remain unidentified. The incidence of the four antibodies provides further links between the various types of connective tissue disease. At the same time, individual connective tissue diseases tend to be associated with particular antibodies. Precipitating factors were detected in the serum of "control" patients with "para- globulinemia", chronic thyroiditis, liver disease, aplastic anemia, and sarcoidosis. The results of precipitin tests are compared with those described by workers using Gadjusek''s "auto-immune complement-fixation reaction" (AlCF), and an explanation of negative autologous tests reported with this latter technique is suggested.