Integrated CMOS photodetectors and signal processing for very low-level chemical sensing with the bioluminescent bioreporter integrated circuit
Integrated CMOS photodetectors and signal processing for very low-level chemical sensing with the bioluminescent bioreporter integrated circuit
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DOI：
10.1016/s0925-4005(02)00106-5
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发表时间：
2002-06-20
影响因子：
8.4
通讯作者：
Simpson, ML
中科院分区：
文献类型：
--
作者：
Bolton, EK;Sayler, GS;Simpson, ML
我们报告了一种针对低水平生物发光检测而优化的集成 CMOS 微发光计，作为生物发光生物报告器集成电路 (BBIC) 的一部分。该微发光计通过仔细管理前端处理电路中流动的亚飞安电流（信号和泄漏），对以前的设备进行了改进。特别是，光电二极管在仅几 mV 的反向偏压下工作，需要特别注意构成前端电路的电流频率转换器 (CFC) 的复位电路。我们使用室温 1.47 mm(2) CMOS 光电二极管报告了亚飞安泄漏电流和 0.15 fA（1510 秒积分时间）的最小可检测信号 (MDS)。该微量发光计可以检测多达 5000 个完全诱导的荧光假单胞菌 5RL 细菌细胞的发光。 (C) 2002 Elsevier Science B.V. 保留所有权利。
We report an integrated CMOS microluminometer optimized for the detection of low-level bioluminescence as part of the bioluminescent bioreporter integrated circuit (BBIC). This microluminometer improves on previous devices through careful management of the sub-femtoampere currents, both signal and leakage, that flow in the front-end processing circuitry. In particular, the photodiode is operated with a reverse bias of only a few mV, requiring special attention to the reset circuitry of the current-to-frequency converter (CFC) that forms the front-end circuit. We report a sub-femtoampere leakage current and a minimum detectable signal (MDS) of 0.15 fA (1510 s integration time) using a room temperature 1.47 mm(2) CMOS photodiode. This microluminometer can detect luminescence from as few as 5000 fully induced Pseudomonas fluorescens 5RL bacterial cells. (C) 2002 Elsevier Science B.V. All rights reserved.