Pulsating fluid flow increases nitric oxide (NO) synthesis by osteocytes but not periosteal fibroblasts - Correlation with prostaglandin upregulation
Pulsating fluid flow increases nitric oxide (NO) synthesis by osteocytes but not periosteal fibroblasts - Correlation with prostaglandin upregulation
复制标题
DOI：
10.1006/bbrc.1995.2822
复制
发表时间：
1995-12-14
影响因子：
3.1
通讯作者：
Burger, EH
中科院分区：
文献类型：
--
作者：
KleinNulend, J;Semeins, CM;Burger, EH
骨细胞对流体剪切应力极其敏感，这种现象可能与骨的机械适应有关（FASEB J 9：441，1995）。在这里，我们检查了脉动流体流（PFF，0.5+/-0.02 Pa，5 Hz，0.4 Pa/sec）对 NO 释放的影响，以及前列腺素 E（2）（PGE（2））上调的关系。鸡颅骨骨细胞（而非骨膜成纤维细胞）以及小鼠颅骨细胞对 PFF 做出反应，快速、短暂地刺激 2 至 3 倍的 NO 释放。 5 分钟后效果达到最大，此后趋于平稳。 PFF 还刺激 PGE(2) 释放。这种效果在 10 分钟后显着，并持续整个 60 分钟的 PFF 治疗。 N-G-单甲基-L-精氨酸对 NO 释放的抑制阻止了 PFF 对 NO 以及 PGE(2) 释放的影响。这些结果表明，NO 是骨中机械效应的介质，导致 PGE(2) 释放增强。他们进一步强化了这样的假设：流经骨细胞小管网络的流体为骨骼中的机械感觉提供了物理刺激。 (C) 1995 学术出版社
Osteocytes are extremely sensitive to fluid shear stress, a phenomenon that may be related to mechanical adaptation of bone (FASEB J 9:441,1995). Here we examined the effect of pulsating fluid flow (PFF, 0.5+/-0.02 Pa, 5 Hz, 0.4 Pa/sec) on the release of NO, in relation with upregulation of prostaglandin E(2) (PGE(2)). Chicken calvarial osteocytes, but not periosteal fibroblasts, as well as mouse calvarial cells responded to PFF with a rapid and transient 2 to 3-fold stimulation of NO release. The effect was maximal after 5 min and leveled off thereafter. PFF also stimulated PGE(2) release. This effect was significant after 10 min and continued throughout 60 min PFF treatment. Inhibition of NO release bq N-G-monomcthyl-L-arginine prevented the effect of PFF on NO as well as PGE(2) release. These results suggest that NO is a mediator of mechanical effects in bone, leading to enhanced PGE(2) release. They further strengthen the hypothesis that fluid flow through the osteocyte canalicular network provides the physical stimulus for mechanosensation in bone. (C) 1995 Academic Press, Inc.