Further characterization of the human clear cell sarcoma cell line HS‐MM demonstrating a specific t(12;22)(q13;q12) translocation and hybrid EWS/ATF‐1 transcript
Further characterization of the human clear cell sarcoma cell line HS‐MM demonstrating a specific t(12;22)(q13;q12) translocation and hybrid EWS/ATF‐1 transcript
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人类透明细胞肉瘤细胞系 HS-MM 的进一步表征，显示特定的 t(12;22)(q13;q12) 易位和混合 EWS/ATF-1 转录本
DOI：
10.1002/(sici)1096-9896(199904)187:5
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Y. Ohtsuki
中科院分区：
文献类型：
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作者：
H. Sonobe;T. Takeuchi;T. Taguchi;Kiichi Shimizu;J. Iwata;M. Furihata;Y. Ohtsuki
仅报道了少量透明细胞肉瘤（CCS）细胞系，包括细胞系 HS-MM。在本研究中，该细胞系自建立以来已维持超过4年，并通过细胞遗传学研究、荧光原位杂交（FISH）分析和逆转录酶聚合酶链反应（RT-PCR）进一步表征。 HS-MM 细胞在体外和体内均表现出具有特定 t(12;22)(q13;q12) 易位的假二倍体核型。通过 FISH 分析证实了 12 号和 22 号染色体之间的易位，并通过 RT-PCR 检测了由该易位诱导的杂合 EWS/ATF-1 转录本。 HS-MM 细胞系将有助于 CCS 的进一步研究。版权所有 © 1999 约翰·威利父子有限公司
Only a small number of clear cell sarcoma (CCS) cell lines have been reported, including the cell line HS‐MM. In the present study, this cell line, maintained for more than 4 years since establishment, was further characterized by cytogenetic studies, fluorescence in situ hybridization (FISH) analysis, and reverse transcriptase‐polymerase chain reaction (RT‐PCR). HS‐MM cells both in vitro and in vivo exhibited pseudodiploid karyotypes with the specific t(12;22)(q13;q12) translocation. The translocation between chromosomes 12 and 22 was confirmed by FISH analysis and the hybrid EWS/ATF‐1 transcript induced by this translocation was detected by RT‐PCR. The HS‐MM cell line will be useful for further studies of CCS. Copyright © 1999 John Wiley & Sons, Ltd.