Biosynthesis of structurally novel carotenoids in Escherichia coli
Biosynthesis of structurally novel carotenoids in Escherichia coli
复制标题
DOI：
10.1016/s1074-5521(03)00103-0
复制
发表时间：
2003-05-01
影响因子：
--
通讯作者：
Schmidt-Dannert, C
中科院分区：
文献类型：
--
作者：
Lee, PC;Momen, AZR;Schmidt-Dannert, C
此前，我们利用体外进化改变了几种类胡萝卜素酶的催化功能，并在大肠杆菌中产生了新型类胡萝卜素四氢番茄红素和甲苯。在此，我们报告了这些途径以及具有额外类胡萝卜素基因的 C-30 胡萝卜素二酮神经孢子烯途径的成功延伸。用 C-40 类胡萝卜素酶（球状烯单加氧酶和番茄红素环化酶）扩展已知的无环 C-30 途径，分别产生新的含氧无环产物和非天然环状 C-30 diapotorulene。用球状烯单加氧酶延伸无环 C-40 途径产生了新型含氧类胡萝卜素，包括紫色菲利普斯黄质。用胡萝卜素羟化酶、去饱和酶、葡糖基酶和酮酶扩展甲苯生物合成途径，产生了新的甲苯衍生物。这些结果证明了利用催化混杂的下游酶扩展体外进化的中心代谢途径以产生结构新颖的化合物的效用。
Previously, we utilized in vitro evolution to alter the catalytic functions of several carotenoid enzymes and produce the novel carotenoids tetradehydrolycopene and torulene in Escherichia coli. Here we report on the successful extension of these pathways and the C-30 carotenold diaponeurosporene pathway with additional carotenoid genes. Extension of the known acyclic C-30 pathway with C-40 carotenoid enzymes-spheroidene monooxygenase and lycopene cyclase-yielded new oxygenated acylic products and the unnatural cyclic C-30 diapotorulene, respectively. Extension of acyclic C-40 pathways with spheroidene monooxygenase generated novel oxygenated carotenoids including the violet phillipsiaxanthin. Extension of the torulene biosynthetic pathway with carotene hydroxylase, desaturase, glucosylase, and ketolase yielded new torulene derivatives. These results demonstrate the utility of extending an in vitro evolved central metabolic pathway with catalytically promiscuous downstream enzymes in order to generate structurally novel compounds.