Assessment of DNA damage in a group of professional dancers during a 10-month dancing season.
Assessment of DNA damage in a group of professional dancers during a 10-month dancing season.
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评估一组专业舞者在 10 个月的舞蹈季节中的 DNA 损伤。
DOI：
10.1080/15287394.2017.1331599
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Costa,Solange
中科院分区：
文献类型：
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作者：
Esteves,Filipa;Teixeira,Eduardo;Amorim,Tânia;Costa,Carla;Pereira,Cristiana;Fraga,Sónia;DeAndrade,VanessaMoraes;Teixeira,JoãoPaulo;Costa,Solange
尽管体育活动对健康有很多好处，但一些研究报告称，增加强度和持续时间可能会导致包括DNA在内的几种细胞成分的氧化应激。这项研究的目的是评估一组专业舞者在10个月的舞蹈季前后的基础DNA损伤水平和氧化DNA损伤水平。一组来自普通人群的个体也被评估为对照。碱性版本的彗星试验是选择测量基础DNA损伤和氧化应激的方法，因为这种方法量化了两个终点。为了测量氧化应激，彗星试验与病变特异性核酸内切酶（甲脒嘧啶糖基化酶）结合检测氧化嘌呤。舞蹈季结束后，舞者体内的氧化性DNA损伤水平显著增加。舞蹈演员的季前氧化DNA损伤水平低于普通人群，这表明舞蹈演员的抗氧化系统具有适应性。目前的生物监测研究结果表明，需要采取更有效的措施来保护芭蕾舞者免受与定期高强度体育锻炼相关的潜在职业健康风险。
Despite the numerous health benefits of physical activity, some studies reported that increased intensity and duration may induce oxidative stress in several cellular components including DNA. The aim of this study was to assess the level of basal DNA damage as well as oxidative DNA damage in a group of professional dancers before and after a 10-month dancing season. A group of individuals from general population was also assessed as a control. The alkaline version of the comet assay was the method selected to measure both basal DNA damage and oxidative stress, since this method quantifies both endpoints. In order to measure oxidative stress, the comet assay was coupled with a lesion-specific endonuclease (formamidopyrimidine glycosylase) to detect oxidized purines. The levels of oxidative DNA damage in dancers were significantly increased after the dancing season. Pre-season levels of oxidative DNA damage were lower in dancers than those obtained from the general population, suggesting an adaptation of antioxidant system in dancers. Results of the present biomonitoring study indicate the need for more effective measures to protect ballet dancers from potentially occupational health risks related to regular intensive physical exercise.