High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy
High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy
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DOI：
10.1038/s41467-018-08216-4
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发表时间：
2019-03-01
影响因子：
16.6
通讯作者：
Loncarek, Jadranka
中科院分区：
文献类型：
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作者：
Bowler, Mathew;Kong, Dong;Loncarek, Jadranka
中心粒是形成中心体和纤毛的重要细胞结构。纤毛的形成和功能依赖于一组中心粒的远端附属物。在这项研究中，我们使用相关的超分辨率和电子显微镜来精确地确定远端附件蛋白相对于中心粒微管和附件电子密度的定位。在这里，我们鉴定了一种新的远端附件蛋白ANKRD26，并以高分辨率详细描述了远端附件组装的初始步骤。我们进一步表明，在有丝分裂之前，远端附属物经历了戏剧性的超结构重组，在此期间，它们暂时失去了外部成分，而内部成分保持了九重组织。最后，利用电子断层扫描，我们发现哺乳动物的远端附属物通过精细的丝状基座与两个中心粒微管三联体相连，它们看起来几乎是放射状的指状突起。我们的发现挑战了哺乳动物远端附肢的传统描述，即在整个有丝分裂过程中保持着风车状的结构。
Centrioles are vital cellular structures that form centrosomes and cilia. The formation and function of cilia depends on a set of centriole's distal appendages. In this study, we use correlative super resolution and electron microscopy to precisely determine where distal appendage proteins localize in relation to the centriole microtubules and appendage electron densities. Here we characterize a novel distal appendage protein ANKRD26 and detail, in high resolution, the initial steps of distal appendage assembly. We further show that distal appendages undergo a dramatic ultra-structural reorganization before mitosis, during which they temporarily lose outer components, while inner components maintain a nine-fold organization. Finally, using electron tomography we reveal that mammalian distal appendages associate with two centriole microtubule triplets via an elaborate filamentous base and that they appear as almost radial finger-like protrusions. Our findings challenge the traditional portrayal of mammalian distal appendage as a pinwheel-like structure that is maintained throughout mitosis.