INVIVO REPLICATION AND REVERSION TO WILD-TYPE OF A NEUTRALIZATION-RESISTANT ANTIGENIC VARIANT OF HEPATITIS-A VIRUS
INVIVO REPLICATION AND REVERSION TO WILD-TYPE OF A NEUTRALIZATION-RESISTANT ANTIGENIC VARIANT OF HEPATITIS-A VIRUS
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DOI：
10.1093/infdis/161.1.7
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发表时间：
1990-01-01
影响因子：
6.4
通讯作者：
LEDUC, JW
中科院分区：
文献类型：
--
作者：
LEMON, SM;BINN, LN;LEDUC, JW
六只血清阴性的猫头鹰猴子静脉注射了甲型肝炎病毒的抗原变体（S18），该病毒高度适应细胞培养中的生长，并且由于衣壳蛋白 VP3 的天冬氨酸 70 被组氨酸取代而抵抗单克隆抗体的中和。接种后 22-33 天，每个人都出现肝炎。通过放射免疫聚焦测定对粪便、血清和肝脏中的病毒进行定量。接种后 7-11 天出现病毒血症，与粪便中病毒排出同时发生，平均持续 20.5 天。尽管从三只动物的粪便或肝脏中回收了抗原变体，但酶升高时肝脏中的病毒主要是野生型抗原表型。攻击后96天仍未从肝脏中回收病毒。这些研究进一步定义了甲型肝炎的病毒学事件，并表明与野生型病毒相比，抗原变体的体内复制受到限制。
Six seronegative owl monkeys were intravenously inoculated with an antigenic variant (S18) of hepatitis A virus that is highly adapted to growth in cell culture and resists neutralization by monoclonal antibodies due to replacement of aspartic acid 70 of capsid protein VP3 with histidine. Each developed hepatitis 22-33 days after inoculation. Virus in feces, serum, and liver was quantified by radioimmunofocus assay. Viremia developed 7-11 days after inoculation, in parallel with fecal shedding of virus, and persisted for a mean of 20.5 days. Although the antigenic variant was recovered from feces or liver of three animals, virus in liver at the time of enzyme elevations was predominantly wild-type antigenic phenotype. Virus was not recovered from liver 96 days after challenge. These studies further define virologic events in hepatitis A and show that in vivo replication of an antigenic variant was restricted compared with that of wild-type virus.