Accumulation of clusterin/sulfated glycoprotein-2 in degenerating pachytene spermatocytes of adult rats treated with methoxyacetic acid.
Accumulation of clusterin/sulfated glycoprotein-2 in degenerating pachytene spermatocytes of adult rats treated with methoxyacetic acid.
复制标题
用甲氧基乙酸处理的成年大鼠退化粗线期精母细胞中凝聚素/硫酸化糖蛋白-2 的积累。
DOI：
10.1095/biolreprod57.4.837
复制
发表时间：
1997
影响因子：
3.6
通讯作者：
Griswold,MD
中科院分区：
文献类型：
--
作者：
Clark,AM;Maguire,SM;Griswold,MD
聚簇蛋白是一种普遍存在的糖蛋白，由支持细胞以相对高的量组成。它与非腺体组织的凋亡、损伤、疾病和修复的关联，使我们研究了聚簇蛋白是否可能是邻近细胞类型凋亡引起的支持细胞损伤诱导反应的一部分。因此，本研究的目的是用甲氧基乙酸（MAA）治疗成年大鼠，选择性地破坏粗线精母细胞，检测睾丸聚集素的定位和表达，并将其与DNA片段化（细胞凋亡的标志）的时间联系起来。maa后6 h，粗线精母细胞的细胞质中存在Clusterin蛋白，而Sertoli细胞中存在Clusterin mRNA。死亡细胞的形态退化和DNA断裂直到12小时才出现。因此，在凋亡过程的早期，支持细胞来源的聚簇蛋白在退化的精母细胞的细胞质中积累。基于这些结果和已知的簇蛋白与疏水大分子的结合，我们假设簇蛋白是由Sertoli细胞产生的，作为一种机制，在精子发生过程中正常发生的生殖细胞退化和膜重塑过程中″清除″潜在的有害细胞成分。
Clusterin is a ubiquitous glycoprotein that is produced constitutively by Sertoli cells at relatively high amounts. Its association with apoptosis, damage, disease, and repair in nongonadal tissues led us to investigate whether clusterin could be part of a damage-induced response in Sertoli cells brought on by apoptosis of an adjacent cell type. Therefore, the objective of this study was to treat adult rats with methoxyacetic acid (MAA) to selectively destroy pachytene spermatocytes, examine the localization and expression of testicular clusterin, and relate this to the timing of DNA fragmentation, a hallmark of apoptosis. Clusterin protein was localized to the cytoplasm of pachytene spermatocytes at 6 h post-MAA, whereas clusterin mRNA was localized to Sertoli cells. Morphological degeneration of dying cells and DNA fragmentation were not seen until 12 h. Thus, Sertoli cell-derived clusterin had accumulated in the cytoplasm of degenerating spermatocytes early in the apoptotic process. On the basis of these results and the known binding of clusterin to hydrophobic macromolecules, we hypothesize that clusterin is produced by Sertoli cells as a mechanism to ″clear″ potentially harmful cellular components during the degeneration of germ cells and remodeling of their membranes that occur normally during spermatogenesis.