The specific guanine binding site of the ribonuclease T1 family enzymes and of G-proteins is modeled in the cocrystal formed by 7-methylguanosine-5'-phosphate and phenylalanine.
The specific guanine binding site of the ribonuclease T1 family enzymes and of G-proteins is modeled in the cocrystal formed by 7-methylguanosine-5'-phosphate and phenylalanine.
复制标题
核糖核酸酶 T1 家族酶和 G 蛋白的特异性鸟嘌呤结合位点在由 7-甲基鸟苷-5-磷酸和苯丙氨酸形成的共晶体中进行建模。
DOI：
10.1093/oxfordjournals.jbchem.a122829
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发表时间：
1989
影响因子：
2.7
通讯作者：
T. Ishida
中科院分区：
文献类型：
--
作者：
W. Saenger;R. Flogel;P. Zielenkiewicz;M. Inoue;T. Ishida
在由7-甲基鸟苷-5 '-磷酸·苯丙氨酸·6 H2O形成的共晶体中，鸟嘌呤和苯丙氨酸之间的相互作用类似于在核糖核酸酶T1与2'-鸟苷酸的复合物中观察到的相互作用，以及两种G-蛋白，延伸因子-Tu和ras癌基因p21与GDP的相互作用。它们在以下三点上是相似的：（a）鸟嘌呤N（1）H和N（2）H提供环N-H; O氢键与前一种共晶体中苯丙氨酸的羧酸基团和后一种蛋白质中Asp或Glu的侧链羧酸基团形成氢键，（B）鸟嘌呤的O（6）接受来自主链NH基团的氢键，（c）嘌呤部分夹在芳香族（或疏水性）氨基酸侧链之间。
In the cocrystal formed by 7-methylguanosine-5'-phosphate.phenylalanine.6H2O, the interactions between guanine and phenylalanine are similar to those observed in the complex of ribonuclease T1 with 2'-guanylic acids, and those of the two G-proteins, Elongation Factor-Tu and ras oncogene p21, with GDP. They are similar in the following three points: (a) guanine N(1)H and N(2)H donate cyclic N-H...O hydrogen bonds to the carboxylate group of phenylalanine in the former cocrystal and to the side chain carboxylate group of Asp or Glu in the latter proteins, (b) O(6) of guanine accepts hydrogen bond(s) from main-chain NH group(s), and (c) the purine moiety is sandwiched between aromatic (or hydrophobic) amino acid side chains.