Response Element-binding Protein ( CREB )-dependent Activation of Per 1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
Response Element-binding Protein ( CREB )-dependent Activation of Per 1 Is Required for Light-induced Signaling in the Suprachiasmatic Nucleus Circadian Clock
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发表时间：
2002
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通讯作者：
S. Tischkau;Jennifer W Mitchell;S. Tyan;G. F. Buchanan;M. Gillette
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作者：
S. Tischkau;Jennifer W Mitchell;S. Tyan;G. F. Buchanan;M. Gillette
光是一个突出的刺激，内源性昼夜节律的环境光/暗周期。夜间光升高Period 1（Per 1）基因的mRNA，并诱导长期的状态变化，表示为昼夜节律的相移。Per 1海拔和光诱导的相位提前在视交叉上核（SCN），一个过程中发起的主要是从retinohypothalamic束的amatergic神经传递的细胞机制，进行了检查。Per 1反义寡核苷酸（ODN）和Ca /cAMP反应元件（CRE）诱饵ODN可阻断谷氨酸（GLU）诱导的大鼠SCN时相提前。CRE-decoy ODN还在体内阻断了光诱导的相位提前。此外，CRE-decoy阻断了Glu诱导的Per 1 mRNA的积累。因此，Ca /cAMP反应元件结合蛋白（CREB）和Per 1是将光刺激的GLU神经传递转换为昼夜节律时钟相位提前的途径的组成部分。
Light is a prominent stimulus that synchronizes endogenous circadian rhythmicity to environmental light/ dark cycles. Nocturnal light elevates mRNA of the Period1 (Per1) gene and induces long term state changes, expressed as phase shifts of circadian rhythms. The cellular mechanism for Per1 elevation and light-induced phase advance in the suprachiasmatic nucleus (SCN), a process initiated primarily by glutamatergic neurotransmission from the retinohypothalamic tract, was examined. Glutamate (GLU)-induced phase advances in the rat SCN were blocked by antisense oligodeoxynucleotide (ODN) against Per1 and Ca /cAMP response element (CRE)-decoy ODN. CRE-decoy ODN also blocked light-induced phase advances in vivo. Furthermore, the CRE-decoy blocked GLU-induced accumulation of Per1 mRNA. Thus, Ca /cAMP response element-binding protein (CREB) and Per1 are integral components of the pathway transducing light-stimulated GLU neurotransmission into phase advance of the circadian clock.