RecA protein filaments disassemble in the 5′ to 3′ direction on single-stranded DNA
RecA protein filaments disassemble in the 5′ to 3′ direction on single-stranded DNA
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DOI：
10.1074/jbc.m109247200
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发表时间：
2001-12-07
影响因子：
4.8
通讯作者：
Inman, RB
中科院分区：
文献类型：
--
作者：
Bork, JM;Cox, MM;Inman, RB
RecA蛋白在单链和双链DNA上形成细丝。几项研究证实，在单链DNA上，丝延伸发生在5'至3'方向。这些细丝也以依赖于末端的方式分解，一些间接的观察表明，分解发生在与组装发生的末端相反的末端。通过用一段双链DNA标记单链DNA的5'端，我们明确地证明了RecA细丝在5'到3'方向上唯一地分解。
RecA protein forms filaments on both single- and double-stranded DNA. Several studies confirm that filament extension occurs in the 5' to 3' direction on single-stranded DNA. These filaments also disassemble in an end-dependent fashion, and several indirect observations suggest that the disassembly occurs on the end opposite to that at which assembly occurs. By labeling the 5' end of single-stranded DNA with a segment of duplex DNA, we demonstrate unambiguously that RecA filaments disassemble uniquely in the 5' to 3' direction.