Immunization with a Pseudomonas aeruginosa elastase peptide reduces severity of experimental lung infections due to P-aeruginosa or Burkholderia cepacia
Immunization with a Pseudomonas aeruginosa elastase peptide reduces severity of experimental lung infections due to P-aeruginosa or Burkholderia cepacia
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DOI：
10.1086/315470
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发表时间：
2000-05-01
影响因子：
6.4
通讯作者：
Woods, DE
中科院分区：
文献类型：
--
作者：
Sokol, PA;Kooi, C;Woods, DE
铜绿假单胞菌和洋葱伯克霍尔德菌产生影响肺损伤的金属蛋白酶。先前在铜绿假单胞菌弹性蛋白酶上鉴定的两个表位（肽15和42）诱导中和蛋白酶活性的抗体的产生。检测了用基于这些表位的合成肽免疫对由于铜绿假单胞菌或洋葱B引起的实验性肺部感染的影响。在感染前用与钥孔血蓝蛋白或破伤风螺旋体缀合的肽免疫大鼠。与对照组相比，用肽15（pep 15）免疫导致支气管肺泡灌洗液（BAL）中的总细胞和多形核白细胞减少，肺组织病理学改变减少50%-70%。用肽42免疫减少BAL液中的细胞，但不减少肺病理变化。单独使用pep 15进行免疫在保护肺损伤方面与使用两种肽的组合进行免疫一样有效。这些研究表明，用pep 15免疫可以降低由于铜绿假单胞菌或洋葱B菌引起的肺部感染的严重程度。
Pseudomonas aeruginosa and Burkholderia cepacia produce metalloproteases that effect lung injury. Two epitopes (peptides 15 and 42) previously identified on P. aeruginosa elastase induce the production of antibodies that neutralize protease activity, The effects of immunization with synthetic peptides based on these epitopes on experimental lung infections due to P. aeruginosa or B, cepacia were examined. Rats were immunized with peptides conjugated to keyhole limpet hemocyanin or tetanus toroid before infection. Immunization with peptide 15 (pep15) resulted in a decrease in total cells and polymorphonuclear leukocytes in bronchoalveolar lavage (BAL) fluid and a 50%-70% decrease in lung histopathologic changes, compared with findings in controls. Immunization with peptide 42 decreased cells in BAL fluid but did not decrease lung pathologic changes. Immunization with pep15 alone was just as effective in protecting against lung injury as immunization with a combination of both peptides. These studies suggest that immunization with pep15 can reduce the severity of lung infections due to P. aeruginosa or B, cepacia.