Sustainable production of biomass and industrially important secondary metabolites in cell cultures of selfheal (Prunella vulgaris L.) elicited by silver and gold nanoparticles
Sustainable production of biomass and industrially important secondary metabolites in cell cultures of selfheal (Prunella vulgaris L.) elicited by silver and gold nanoparticles
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DOI：
10.1080/21691401.2019.1625913
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发表时间：
2019-01-01
影响因子：
5.8
通讯作者：
Wei, Dong-Qing
中科院分区：
文献类型：
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作者：
Fazal, Hina;Abbasi, Bilal Haider;Wei, Dong-Qing
诱导型人工体外培养因其能均匀合成具有工业价值的次生代谢物而受到越来越多的关注。在这项研究中，采用了一种独特的方法，在液体深层培养中添加不同比例的金(Au)和银(Ag)纳米颗粒(NPs)来研究生物量和抗氧化剂次生代谢产物的可持续生产。将细胞悬液单独暴露于Ag和AuNPs，或不同比例的AgAuNPs(1：2；1：3；2：1；3：1)联合NAA。AgAuNPs与NAA以3：1的比例组合后，悬浮细胞的鲜重(9.25g/100ml)和干重(0.64g/100ml)均高于对照组(6.67和0.233g/100ml)。添加NAA的AuNPs在49d的周期内促进了滞后期、对数期和稳定期的生物量积累。此外，添加NAA的AgAU(3：1)和AgAuNPs(2：1；1：2)提高了蛋白质含量、过氧化物酶和超氧化物歧化酶活性。然而，在AgAuNPs(1：3)和NAA的联合作用下，酚类化合物(TPC；10.61 mg/g-DW)和类黄酮类化合物(7.62 mg/g-DW)明显高于对照组(6.27，5.49 mg/g-DW)。在细胞培养中，AgAuNPs(2：1)和NAA的结合提高了细胞的抗氧化活性(87.85%)。这项研究将有助于阐明NPs对培养、发育和生产天然抗氧化剂的影响。
Elicited artificial in vitro cultures are gaining more interest due to their uniform biosynthesis of industrially valuable secondary metabolites. In this study, a unique methodology was applied, in which different ratios of gold (Au) and silver (Ag) nanoparticles (NPs) were supplemented to submerge cultures to investigate sustainable production of biomass and antioxidant secondary metabolites. Cell suspension cultures were exposed to Ag and AuNPs alone or different ratios of AgAuNPs (1:2; 1:3; 2:1; 3:1) in combination with NAA. The combination of AgAuNPs (3:1) with NAA enhanced fresh (9.25 g/100 ml) and dry biomass (0.64 g/100 ml) of suspended cells than control (6.67; 0.233g/100 ml). AuNPs with NAA-augmented media enhanced biomass accumulation in lag, log and stationary phases in a period of 49days. Furthermore, AgAu (3:1) and AgAuNPs (2:1; 1:2) with NAA enhanced protein contents, peroxidase and superoxide dismutase enzymes. However, maximum phenolics (TPC; 10.61 mg/g-DW) and flavonoids (7.62 mg/g-DW) were observed in cell cultures exposed to a combination of AgAuNPs (1:3) and NAA than control (6.27, 5.49 mg/g-DW). The combination of AgAuNPs (2:1) with NAA enhanced antioxidant activity (87.85%) in cell cultures. This study will help in illuminating the impact of NPs on cultures development and production of natural antioxidants.