Molecular genotyping of human Ureaplasma species based on multiple-banded antigen (MBA) gene sequences.
Molecular genotyping of human Ureaplasma species based on multiple-banded antigen (MBA) gene sequences.
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基于多带抗原 (MBA) 基因序列的人类解脲支原体物种的分子基因分型。
DOI：
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发表时间：
2000
影响因子：
2.8
通讯作者：
G. Gilbert
中科院分区：
文献类型：
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作者：
F. Kong;Z. Ma;G. James;S. Gordon;G. Gilbert
解脲脲原体分为14个血清型。近年来，美国的细分市场。Urealyticum分为两个种：U. parvum（以前的U.解脲脲原体（Urealyticum parvo biovar），包括4个血清型（1，3，6，14）和解脲脲原体（U.解脲脲原体（Urealyticum）解脲支原体T-960生物型），10个血清型（2、4、5、7-13）。这些物种的多带抗原（MBA）基因包含物种和血清型/亚型特异性序列。根据U.小乌藨子（U. parvum）血清型和MBA基因5 ′端的部分序列。对于解脲支原体血清型，我们先前将这些物种中的每一个分为三种MBA基因型。为了进一步阐明血清型之间的关系，我们对所有10个U.解脲脲原体各血清型的基因序列及部分重复序列。parvum和U.溶脲菌这是第一次，所有四个血清型的U。parvum明显区分彼此。此外，U.将解脲支原体分为5种MBA基因型，如下所示：MBA基因型A包括血清型2、5、8; MBA基因型B，仅血清型10; MBA基因型C，血清型4、12、13; MBA基因型D，仅血清型9; MBA基因型E包括血清型7和11。每个MBA基因型内的成员之间没有序列差异。需要进一步的工作来鉴定其他基因或MBA基因的其他区域，这些基因可能用于区分U。MBA基因型A、C和E内的解脲支原体血清型。更好地了解血清型分化的分子基础将有助于改进亚型分型方法，用于这些生物体的发病机制和流行病学研究。
Ureaplasma urealyticum has been divided into 14 serovars. Recently, subdivision of U. urealyticum into two species has been proposed: U. parvum (previously U. urealyticum parvo biovar), comprising four serovars (1, 3, 6, 14) and U. urealyticum (previously U. urealyticum T-960 biovar), 10 serovars (2, 4, 5, 7-13). The multiple-banded antigen (MBA) genes of these species contain both species and serovar/subtype specific sequences. Based on whole sequences of the 5'-ends of MBA genes of U. parvum serovars and partial sequences of the 5'-ends of MBA genes of U. urealyticum serovars, we previously divided each of these species into three MBA genotypes. To further elucidate the relationships between serovars, we sequenced the whole 5'-ends of MBA genes of all 10 U. urealyticum serovars and partial repetitive regions of these genes from all serovars of U. parvum and U. urealyticum. For the first time, all four serovars of U. parvum were clearly differentiated from each other. In addition, the 10 serovars of U. urealyticum were divided into five MBA genotypes, as follows: MBA genotype A comprises serovars 2, 5, 8; MBA genotype B, serovar 10 only; MBA genotype C, serovars 4, 12, 13; MBA genotype D, serovar 9 only; and MBA genotype E comprises serovars 7 and 11. There were no sequence differences between members within each MBA genotype. Further work is required to identify other genes or other regions of the MBA genes that may be used to differentiate U. urealyticum serovars within MBA genotypes A, C and E. A better understanding of the molecular basis of serotype differentiation will help to improve subtyping methods for use in studies of the pathogenesis and epidemiology of these organisms.