Exercise induces expression of leukaemia inhibitory factor in human skeletal muscle
Exercise induces expression of leukaemia inhibitory factor in human skeletal muscle
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DOI：
10.1113/jphysiol.2007.149781
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发表时间：
2008-04-15
影响因子：
5.5
通讯作者：
Pedersen, Bente Klarlund
中科院分区：
文献类型：
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作者：
Broholm, Christa;Mortensen, Ole Hartvig;Pedersen, Bente Klarlund
白血病抑制因子（LIF）属于白细胞介素(IL)-6细胞因子超家族，在骨骼肌中组成性表达。我们验证了人体骨骼肌中LIF表达受运动调节的假设。15名健康的年轻男性志愿者分别进行了3小时的自行车运动（n = 8）和休息（n = 7）。在运动前、运动后以及运动后1.5、3、6和24小时分别对股外侧肌进行肌肉活检。对照受试者在运动试验期间的同一时间点采集活检样本。骨骼肌LIF mRNA在运动后立即升高，在恢复过程中逐渐下降。然而，在研究的时间点上，LIF蛋白不变。此外，我们验证了在原代人骨骼肌细胞中LIF mRNA和蛋白表达受钙（Ca2+）调节的假设。用Ca2+离子载体（离子霉素）处理肌细胞6小时，导致LIF mRNA和LIF蛋白水平升高。这一发现表明Ca2+可能参与了耐力运动骨骼肌中LIF的调节。综上所述，人原代骨骼肌细胞在离子霉素刺激下具有产生LIF的能力，同心圆运动后骨骼肌中LIF mRNA水平升高。研究发现，骨骼肌LIF蛋白并没有随着LIF mRNA水平的增加而增加，这表明，为了诱导LIF蛋白的可检测积累，其他运动刺激或重复刺激是必要的。
The leukaemia inhibitory factor (LIF) belongs to the interleukin (IL)-6 cytokine superfamily and is constitutively expressed in skeletal muscle. We tested the hypothesis that LIF expression in human skeletal muscle is regulated by exercise. Fifteen healthy young male volunteers performed either 3 h of cycle ergometer exercise at similar to 60% of (n = 8) or rested (n = 7). Muscle biopsies were obtained from the vastus lateralis prior to exercise, immediately after exercise, and at 1.5, 3, 6 and 24 h post exercise. Control subjects had biopsy samples taken at the same time points as during the exercise trial. Skeletal muscle LIF mRNA increased immediately after the exercise and declined gradually during recovery. However, LIF protein was unchanged at the investigated time points. Moreover, we tested the hypothesis that LIF mRNA and protein expressions are modulated by calcium (Ca2+) in primary human skeletal myocytes. Treatment of myocytes with the Ca2+ ionophore, ionomycin, for 6 h resulted in an increase in both LIF mRNA and LIF protein levels. This finding suggests that Ca2+ may be involved in the regulation of LIF in endurance-exercised skeletal muscle. In conclusion, primary human skeletal myocytes have the capability to produce LIF in response to ionomycin stimulation and LIF mRNA levels increase in skeletal muscle following concentric exercise. The finding that the increase in LIF mRNA levels is not followed by a similar increase in skeletal muscle LIF protein suggests that other exercise stimuli or repetitive stimuli are necessary in order to induce a detectable accumulation of LIF protein.