Molecular detection and characterization of genotype 1 bovine leukemia virus from beef cattle in the traditional sector in Zambia
Molecular detection and characterization of genotype 1 bovine leukemia virus from beef cattle in the traditional sector in Zambia
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DOI：
10.1007/s00705-019-04350-6
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发表时间：
2019-10-01
影响因子：
2.7
通讯作者：
Simulundu, Edgar
中科院分区：
文献类型：
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作者：
Phiri, Mundia M.;Kaimoyo, Evans;Simulundu, Edgar
虽然牛白血病病毒(BLV)给世界各地的奶牛业造成了巨大的经济损失，但关于其分子流行病学和对肉牛的经济影响的信息有限。在这里，来自赞比亚主要养牛省份的880只动物的血液被用套式聚合酶链式反应进行BLV筛查。对阳性池进行了测序和系统发育分析。估计合并患病率为2.1%。所有菌株都属于基因1，并形成了一个明显的系统发育簇。这项研究表明，BLV基因1型在这些地区的肉牛中传播。这是非洲首次对肉牛BLV进行分子检测和鉴定。
Whilst bovine leukemia virus (BLV) causes considerable economic losses to the dairy industry worldwide, information on its molecular epidemiology and economic impact in beef cattle is limited. Here, blood from 880 animals from Zambia's major cattle-rearing provinces was screened for BLV by nested PCR. Positive pools were sequenced and phylogenetically analyzed. The estimated pooled prevalence was 2.1%. All strains belonged to genotype 1 and formed a distinct phylogenetic cluster. The study suggests circulation of genotype 1 BLV in beef cattle in these regions. This is the first report on molecular detection and characterization of BLV from beef cattle in Africa.