IDENTIFICATION OF SKELETAL-MUSCLE PRECURSOR CELLS INVIVO BY USE OF MYOD1 AND MYOGENIN PROBES
IDENTIFICATION OF SKELETAL-MUSCLE PRECURSOR CELLS INVIVO BY USE OF MYOD1 AND MYOGENIN PROBES
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DOI：
10.1007/bf00318695
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发表时间：
1992-01-01
影响因子：
3.6
通讯作者：
BEILHARZ, MW
中科院分区：
文献类型：
--
作者：
GROUNDS, MD;GARRETT, KL;BEILHARZ, MW
用MyoD1和myogenin探针原位杂交检测小鼠胫骨前肌挤压伤后单核肌前体细胞的活化。 这些基因是骨骼肌分化的早期标志物，并已在体外进行了广泛的研究。 这些调节蛋白在体内成熟肌肉的肌发生过程中的作用以前没有研究过。 MyoD1和肌细胞生成素mRNA存在于未损伤肌肉的偶尔单个核细胞中。 单核细胞中MyoD1和myoigenin mRNA序列的增加早在损伤后6小时就被检测到，在24和48小时之间达到峰值，此后在约8天下降到损伤前水平。 在整个肌肉的单核细胞中检测到mRNA，大多数细胞位于距离挤压损伤部位一定距离处。 MyoD1和肌细胞生成素mRNA在6至48小时的存在表明这些基因的转录与体内肌肉前体细胞的复制同时发生。 在任何时候都没有显着水平的mRNA，这些基因检测肌管。 MyoD1和myogenin为骨骼肌前体细胞的早期鉴定和研究提供了精确的标记。
The activation of mononuclear muscle precursor cells after crush injury to mouse tibialis anterior muscles was monitored in vivo by in situ hybridization with MyoD1 and myogenin probes. These genes are early markers of skeletal muscle differentiation and have been extensively studied in vitro. The role in vivo of these regulatory proteins during myogenesis of mature muscle has not been studied previously. MyoD1 and myogenin mRNA were present in occasional mononuclear cells of uninjured muscle. Increased MyoD1 and myoigenin mRNA sequences in mononuclear cells were detected as early as 6 h after injury, peaked between 24 and 48 h, and thereafter decline to pre-injury levels at about 8 days. The mRNAs were detected in mononuclear cells throughout the muscle, with the majority of cells located some distance from the site of crush injury. The presence of MyoD1 and myogenin mRNA at 6 to 48 h indicates that transcription of these genes is occurring at the same time as replication of muscle precursor cells in vivo. At no time were significant levels of mRNA for these genes detected in myotubes. MyoD1 and myogenin provide precise markers for the very early identification and study of mononuclear skeletal muscle precursor cells in muscle regenerating in vivo.