Active promoters and insulators are marked by the centrosomal protein 190
Active promoters and insulators are marked by the centrosomal protein 190
复制标题
DOI：
10.1038/emboj.2009.34
复制
发表时间：
2009-04-08
期刊：
影响因子：
11.4
通讯作者：
Renkawitz, Rainer
中科院分区：
文献类型：
--
作者：
Bartkuhn, Marek;Straub, Tobias;Renkawitz, Rainer
对于紧凑的果蝇基因组，已经确定了几个调节绝缘体功能的因子，如su(HW)和dCTCF。最近的分析表明，这两种绝缘子结合因子在功能上都依赖于相同的辅因子CP190。在这里，我们分析了CP190和dCTCF的全基因组结合。CP190结合在ctcf、su(Hw)和GAF位点，并意外地出现在活跃转录基因的转录起始点。绝缘体和转录起始点CP190结合元件严格标记为组蛋白H3的耗尽，因此，核小体占有率的丧失。此外，在许多H3K27me3“岛屿”的边界上也看到了CP190/dCTCF双重入住率。像以前一样，这些地点也耗尽了H3。DCTCF或CP190的缺失会导致这些位点上H3和H3K27三甲基化的增加。因此，对于两种类型的顺式调控元件、结构域边界和启动子，染色质结构都依赖于CP190。
For the compact Drosophila genome, several factors mediating insulator function, such as su(Hw) and dCTCF, have been identified. Recent analyses showed that both these insulator-binding factors are functionally dependent on the same cofactor, CP190. Here we analysed genome-wide binding of CP190 and dCTCF. CP190 binding was detected at CTCF, su( Hw) and GAF sites and unexpectedly at the transcriptional start sites of actively transcribed genes. Both insulator and transcription start site CP190-binding elements are strictly marked by a depletion of histone H3 and, therefore, a loss of nucleosome occupancy. In addition, CP190/dCTCF double occupancy was seen at the borders of many H3K27me3 'islands'. As before, these sites were also depleted of H3. Loss of either dCTCF or CP190 causes an increase of H3 and H3K27 trimethylation at these sites. Thus, for both types of cis-regulatory elements, domain borders and promoters, the chromatin structure is dependent on CP190.