Genome-Scale Expression Pattern of Long Non-Coding RNAs in Chinese Uyghur Patients with Parkinson's Disease.
Genome-Scale Expression Pattern of Long Non-Coding RNAs in Chinese Uyghur Patients with Parkinson's Disease.
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DOI：
10.12659/msm.925888
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发表时间：
2020-10-08
期刊：
影响因子：
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通讯作者：
Yang X
中科院分区：
文献类型：
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作者：
Wang D;Gao H;Li Y;Jiang S;Yong Y;Yang X
长非编码RNA(LncRNAs)是被认为在转录后水平上调节基因表达的转录本。一些lncRNAs与帕金森病相关，参与帕金森病的病理过程。新疆中国地区维吾尔族帕金森病的发病率较高。本研究检测了中国维吾尔族帕金森病患者和非帕金森病患者外周血细胞中lncRNAs的表达，并分析了这些lncRNAs在帕金森病发生发展中的可能作用。采集55例维吾尔族帕金森病患者和55例健康志愿者的外周血。提取总RNA，用基因芯片技术检测10例PD和5例正常对照的全基因组lncRNAs和mRNAs的表达水平。采用定量逆转录聚合酶链式反应(qRT-PCR)检测100例受试者中LncRNAs的表达水平。采用基因芯片技术检测PD患者的LncRNA表达谱，并对差异表达的LncRNA进行鉴定。芯片分析的结果在大量临床队列中得到了证实。比较有无帕金森病的受试者，发现帕金森病组中32个显著上调，18个显著下调。GO分析表明，参与生物过程调控的编码蛋白的mRNAs存在差异表达，其中炎性免疫反应是最相关的途径。PD患者和正常对照组外周血中LncRNAs的表达差异有统计学意义。这些差异表达的lncRNAs可能在帕金森病的发生发展中起一定作用。
Long non-coding RNAs (lncRNAs) are transcripts thought to regulate gene expression at the post-transcriptional level. Some lncRNAs are associated with Parkinson’s disease (PD) and participate in pathological processes of PD. The incidence of PD is relatively high in members of the Uyghur minority living in Xingjiang province of China. This study measured the expression of lncRNAs in the peripheral blood cells of Chinese Uyghur individuals with and without PD and analyzed the possible function of these lncRNAs in the development of PD. Peripheral blood samples were collected from 55 Uyghur patients with PD and 55 healthy volunteers. Total RNA was extracted, and the levels of expression of whole-genome lncRNAs and mRNAs in 10 samples (5 PD and 5 controls) were determined by microarray method. The expression levels of lncRNAs in all 100 subjects were determined by qRT-PCR. The lncRNA expression profiles of PD patients were determined based on lncRNA microarray chip analysis, and differentially expressed lncRNAs were identified. The results of chip analysis were confirmed in a large clinical cohort. Comparison of subjects with and without PD identified 32 significantly up-regulated and 18 significantly down-regulated lncRNAs in the PD group. GO analysis showed that mRNAs encoding proteins involved in the regulation of biological processes were differentially expressed, with the inflammatory immune response being the most significantly related pathway. The expression of lncRNAs in peripheral blood differed significantly in PD patients and controls. These differentially expressed lncRNAs may play a role in the development of PD.