Radioimmunoassay of bile acids in serum.
Radioimmunoassay of bile acids in serum.
复制标题
血清中胆汁酸的放射免疫测定。
作者：
L. Demers;G. Hepner
我们建立了四种测定血清中甘氨酸结合胆汁酸的放射免疫分析方法。用碳化二亚胺反应将两种主要胆汁酸(胆汁酸和鹅去氧胆酰甘氨酸)和两种次要胆汁酸(硫代胆汁酸和脱氧胆酰甘氨酸)通过碳二亚胺反应与白蛋白共价连接后，在兔体内产生抗体。每种胆汁酸的测定灵敏度都在皮摩尔范围内，标准曲线从10-80pmol延伸。血清胆汁酸浓度在肝病的不同状态下升高，其测量似乎是肝功能的一个极其敏感的指标。
We developed four radioimmunoassay procedures for the determination of glycine-conjugated bile acids in serum. Antibodies to two primary bile acids, cholylgycine and chenodeoxycholylglycine, and to two secondary bile acids, sulfolithocholylglycine and deoxycholylglycine, were raised in rabbits after the acids were covalently linked to albumin by use of the carbodiimide reaction. Assay sensitivity for each of these bile acids is in the picomole range with the standard curve extending from 10-80 pmol. The concentration of bile acids in serum increased in various states of liver disease and its measurement appears to be an extremely sensitive indicator of liver function.