Characterization of the protective response against a homologous challenge infection with Strongyloides venezuelensis in rats
Characterization of the protective response against a homologous challenge infection with Strongyloides venezuelensis in rats
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DOI：
10.1016/s0304-4017(03)00085-2
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发表时间：
2003-05-01
影响因子：
2.6
通讯作者：
Kakoma, I
中科院分区：
文献类型：
--
作者：
Baek, BK;Islam, MK;Kakoma, I
研究了大鼠对同源攻击感染文氏圆线虫的保护性反应。在首次感染L 3号丝状幼虫和迁徙幼虫时，L 3号在感染后第3天肺中的数量为180.8±/-14.5，在小肠中的成虫数量在第7天为336.8+/-70.7.后者在第42天逐渐被排出体外。在初始感染后，大鼠对同源攻击感染产生了很强的免疫力，表现为攻击后第14天，蠕虫数量显著减少，体长和体宽发育迟缓，生殖器官受损，产卵量减少，虫子迅速排出。在驱除蠕虫之前，外周血嗜酸性粒细胞计数显著升高，无论是初始(200.0+/-26.5x10(3)ml)还是攻击感染(400.9+/-165.4 x10(3)ml)。这些发现表明，大鼠在最初暴露于金黄色葡萄球菌后获得了较强的同源免疫。提示PBE参与了蠕虫的驱除。这些效应机制的一个主要靶点是文氏链霉菌的生殖系统。(C)2003 Elsevier Science B.V.保留所有权利。
The protective response in rats against a homologous challenge infection with Strongyloides venezuelensis was characterized. In an initial infection with 1000 filariform larvae and migrating larvae (L-3) of S. venezuelensis, the population of L-3 in the lungs on day 3 postinfection (PI), and that of adult worms in the small intestine on day 7 PI, were 180.8 +/- 14.5 and 336.8 +/- 70.7, respectively. The latter were gradually expelled towards day 42 PI. After the initial infection, the rats developed strong immunity against a homologous challenge infection as manifested by a marked reduction in worm populations, stunted body length and width, damage to reproductive organs, impaired egg production and rapid expulsion of the worms by day 14 after challenge. Expulsion of the worms was preceded by a significantly elevated (P < 0.05) peripheral blood eosinophil (PBE) count, both in the initial (200.0 +/- 26.5 x 10(3) ml) and the challenge infection (400.9 +/- 165.4 x 10(3) ml). These findings suggest that rats acquire strong homologous immunity following initial exposure to S. venezuelensis. It is suggested that PBEs are involved in worm expulsion. A major target of these effector mechanisms is the reproductive system of S. venezuelensis. (C) 2003 Elsevier Science B.V. All rights reserved.