MCRA AND MCRB RESTRICTION PHENOTYPES OF SOME ESCHERICHIA-COLI STRAINS AND IMPLICATIONS FOR GENE CLONING
MCRA AND MCRB RESTRICTION PHENOTYPES OF SOME ESCHERICHIA-COLI STRAINS AND IMPLICATIONS FOR GENE CLONING
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DOI：
10.1093/nar/16.4.1563
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发表时间：
1988-02-25
影响因子：
14.9
通讯作者：
HANAHAN, D
中科院分区：
文献类型：
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作者：
RALEIGH, EA;MURRAY, NE;HANAHAN, D
对E.大肠杆菌干扰含有甲基胞嘧啶的DNA。来自许多生物体的DNA，包括所有哺乳动物和植物的DNA，预计是敏感的，这可能会干扰克隆实验。先前已报道了少数菌株的McrA和B表型（1 - 4）。对94株主要来源于大肠杆菌的菌株进行了Mcr表型分析。大肠杆菌K12，列表如下。我们简要回顾了一些证据表明，小鼠修饰的DNA的McrB限制确实发生在体内，事实上干扰特定小鼠序列的克隆。
The McrA and McrB (modified cytosine restriction) systems of E. coli interfere with incoming DNA containing methylcytosine. DNA from many organisms, including all mammalian and plant DNA, is expected to be sensitive, and this could interfere with cloning experiments. The McrA and B phenotypes of a few strains have been reported previously (1-4). The Mcr phenotypes of 94 strains, primarily derived from E. coli K12, are tabulated here. We briefly review some evidence suggesting that McrB restriction of mouse-modified DNA does occur in vivo and does in fact interfere with cloning of specific mouse sequences.