Distinguishing Effects of Ultraviolet Exposure and Chlorination on the Horizontal Transfer of Antibiotic Resistance Genes in Municipal Wastewater
Distinguishing Effects of Ultraviolet Exposure and Chlorination on the Horizontal Transfer of Antibiotic Resistance Genes in Municipal Wastewater
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DOI：
10.1021/acs.est.5b00644
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发表时间：
2015-05-05
影响因子：
11.4
通讯作者：
Yang, Jian
中科院分区：
文献类型：
--
作者：
Guo, Mei-Ting;Yuan, Qing-Bin;Yang, Jian
抗生素抗性基因（ARGs）在废水微生物群落中的传播越来越受到关注;然而，作为微生物控制技术的消毒过程尚未评估其对ARGs转移的影响。本研究以革兰氏阴性大肠杆菌菌株间的接合转移模型为基础，探讨了紫外线消毒和氯化消毒对ARGs转移频率的影响。杆菌结果表明，紫外线消毒和氯化处理对接合转移有明显的影响。低剂量的紫外线（高达8 mJ/cm（2））对接合转移频率的影响很小，紫外线暴露仅减少细菌数量，但不改变细胞渗透性。相比之下，低氯剂量（高达40毫克氯分钟/L）显着促进接合转移的频率2-5倍。产生的氯胺刺激细菌并提高细胞通透性。接合细胞表面产生较多的纤毛，为ARGs的转运提供了途径。高剂量的紫外线（>10 mJ/cm（2））或氯（>80 mg Cl min/L）可显著抑制ARG转移的频率。
Growing attention has been paid to the dissemination of antibiotic resistance genes (ARGs) in wastewater microbial communities; however, the disinfection processes, as microbial control technologies, have not been evaluated for their impacts on ARGs transfer. In this study, the effects of ultraviolet (UV) disinfection and chlorination on the frequency of ARGs transfer have been explored based on the conjugative transfer model between Gram-negative strains of E. coli. The results indicated that UV disinfection and chlorination exhibit distinct influences on the conjugative transfer. Low UV doses (up to 8 mJ/cm(2)) had little influence on the frequency of conjugative transfer, and UV exposure only decreased the bacterial number but did not change the cell permeability. By comparison, low chlorine doses (up to 40 mg Cl min/L) significantly promoted the frequency of conjugative transfer by 2-5-fold. The generated chloramine stimulated the bacteria and improved the cell permeability. More pilus were induced on the surface of conjugative cells, which acted as pathways for ARGs transfer. The frequency of ARG transfers was greatly suppressed by high doses of UV (>10 mJ/cm(2)) or chlorine (>80 mg Cl min/L).