The importance of G1/S-border and mitosis in the fixation of potentially lethal damage
The importance of G1/S-border and mitosis in the fixation of potentially lethal damage
复制标题
G1/S 边界和有丝分裂在固定潜在致命损伤中的重要性
DOI：
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发表时间：
1983
影响因子：
1.7
通讯作者：
M. Nüsse
中科院分区：
文献类型：
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作者：
G. Iliakis;M. Nüsse
通过在给予辐射的同一阶段或随后的阶段中引入细胞周期进展的延迟来测量同步化埃利希腹水肿瘤细胞修复PLD的能力。为此目的，将细胞在平衡盐溶液中孵育，其非特异性地延迟所有细胞周期阶段的进展，或与0.5 μg/ml阿非迪霉素一起孵育，其延迟细胞处于S期。在细胞周期中进展延迟的细胞能够修复PLD，而不管它们被保持在哪个阶段。在通过细胞周期的进展中的延迟的情况下，在暴露于辐射之后的阶段中引入，仅当细胞没有通过G1/S-边界或有丝分裂时才观察到PLD的修复。根据这些结果，G1/S-边界和有丝分裂在PLD固定中的重要性被提出。
SummaryThe ability of synchronized Ehrlich ascites tumour cells to repair PLD was measured by introducing delays in their progression through the cell cycle either in the same phase as that where the irradiation was given or in a subsequent phase. Cells were incubated for this purpose either in balanced salt solution which nonspecifically delayed progression in all cell cycle phases or with 0.5 µg/ml aphidicolin which delayed cells in S-phase. Cells which had been delayed in their progression through the cell cycle were able to repair PLD irrespective of the phase at which they were held. In cases where the delay in the progression through the cell cycle was introduced in a phase subsequent to that of the exposure to irradiation, repair of PLD was observed only if the cells had not passed the G1/S-border or mitosis. Based on these results, the importance of G1/S-border and mitosis in the fixation of PLD is suggested.