Time scales of membrane fusion revealed by direct imaging of vesicle fusion with high temporal resolution
Time scales of membrane fusion revealed by direct imaging of vesicle fusion with high temporal resolution
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DOI：
10.1073/pnas.0602766103
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发表时间：
2006-10-24
影响因子：
11.1
通讯作者：
Dimova, Rumiana
中科院分区：
文献类型：
--
作者：
Haluska, Christopher K.;Riske, Karin A.;Dimova, Rumiana
膜融合是生命的重要过程，例如，通过胞吐作用参与细胞分泌、神经细胞之间的信号传导和病毒感染。在生命科学和生物工程中，受控膜融合有许多可能的应用，如药物输送，基因转移，化学微反应器或纳米材料的合成。到目前为止，聚变动力学一直难以捉摸，因为直接观测仅限于超过几毫秒的时间尺度。在这里，巨大的脂质囊泡的融合是以受控的方式诱导的，并以50 μ s的时间分辨率进行监测。使用基于合成的融合分子和电穿孔的两种不同的融合方案。对于这两种方案，融合颈的打开非常快，平均扩张速度为厘米/秒。这一速度表明，单个熔合颈的初始形成可以在几百纳秒内完成。
Membrane fusion is a vital process of life involved, for example, in cellular secretion via exocytosis, signaling between nerve cells, and virus infection. In both the life sciences and bioengineering, controlled membrane fusion has many possible applications, such as drug delivery, gene transfer, chemical microreactors, or synthesis of nanomaterials. Until now, the fusion dynamics has been elusive because direct observations have been limited to time scales that exceed several milliseconds. Here, the fusion of giant lipid vesicles is induced in a controlled manner and monitored with a temporal resolution of 50 mu s. Two different fusion protocols are used that are based on synthetic fusogenic molecules and electroporation. For both protocols, the opening of the fusion necks is very fast, with an average expansion velocity of centimeters per second. This velocity indicates that the initial formation of a single fusion neck can be completed in a few hundred nanoseconds.