Use of consensus sequences and genetic networks to identify members of the Bemisia tabaci cryptic species complex in Egypt and Syria
Use of consensus sequences and genetic networks to identify members of the Bemisia tabaci cryptic species complex in Egypt and Syria
复制标题
使用共有序列和遗传网络来识别埃及和叙利亚烟粉虱隐秘物种复合体的成员
DOI：
10.1111/j.1439-0418.2011.01673.x
复制
发表时间：
2012-08-01
影响因子：
1.9
通讯作者：
Qiu, B-L
中科院分区：
文献类型：
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作者：
Ahmed, M. Z.;De Barro, P. J.;Qiu, B-L
甘薯粉虱Bemisia tabaci（Gennadius）是一个由至少24个形态上难以区分的种组成的隐种复合体。整个综合体的害虫状况差异很大，有些害虫对杀虫剂产生抗药性的能力也很大，因此，认识到杀虫剂的特性对于制定有效的控制措施至关重要。以前，系统发育重建已被用来确定不同的B。烟粉虱，但这种方法不再是必要的，因为存在的线粒体细胞色素氧化酶一个共同序列的每个已知物种。因此，我们使用这些共有序列来确定叙利亚和埃及复合体成员的身份，然后使用遗传网络来揭示它们与被分配物种中其他单倍型的遗传相关性模式。结果显示，在叙利亚、亚洲II 1、中东-小亚细亚1（这相当于通常称为B生物型的全球入侵者）和地中海（这相当于通常称为Q生物型的全球入侵者）存在三个物种。 埃及有两个神秘的物种，中东-小亚细亚1和地中海。 在叙利亚，中东-小亚细亚1只在大马士革附近（叙利亚西南部）发现，而地中海则在整个阿勒颇（叙利亚北方）和哈马（叙利亚中北部）发现。 AsiaII 1在希姆斯（叙利亚中南部）和大马士革（叙利亚西南部）附近发现。 在埃及，地中海被发现在开罗和伊斯梅利亚（上埃及中部），而中东-小亚细亚1被发现在上埃及，苏伊士，北西奈，塞德港，达卡希拉，贝赫拉和亚历山大的其余所有部分，覆盖了埃及的主要农业区。 还讨论了叙利亚和埃及人口彼此之间以及与世界其他地区的遗传相关性。
The sweet potato whitefly, Bemisia tabaci (Gennadius), is a cryptic species complex composed of at least 24 different morphologically indistinguishable species. The considerable differences in the pest status across the complex, and the ability of some to develop resistance to, insecticides make awareness of their identity critical in terms of developing effective control measures. Previously, phylogenetic reconstructions have been used to identify different B. tabaci, but this approach is no longer necessary because of the existence of mitochondrial cytochrome oxidase one consensus sequences for each of the known species. We therefore use these consensus sequences to determine the identities of the members of the complex in Syria and Egypt and then used genetic networks to reveal the pattern of their genetic relatedness to other haplotypes in the species to which they were assigned. The results showed the presence of three species in Syria, AsiaII 1, Middle East–Asia Minor 1 (this equates to the global invader known commonly as the B biotype) and Mediterranean (this equates to the global invader known commonly as the Q biotype). Egypt was shown to have two cryptic species, Middle East–Asia Minor 1 and Mediterranean. In Syria, Middle East–Asia Minor 1 was found around Damascus only (south‐west Syria), while Mediterranean was found throughout Aleppo (northern Syria) and Hama (north central Syria). AsiaII 1 was found around Hims (south central Syria) and Damascus (south‐western Syria). In Egypt, Mediterranean was found in Cairo and Ismailia (central Upper Egypt), while Middle East–Asia Minor 1 was found in the remaining all parts of Upper Egypt, Suez, North Sinai, Port Said, Dakahila, Behera and Alexandria which cover the main agricultural zone of Egypt. Genetic relatedness of Syrian and Egyptian populations with each other and with rest of world is also discussed.