A Drosophila Winged‐helix nude (Whn)‐like transcription factor with essential functions throughout development
A Drosophila Winged‐helix nude (Whn)‐like transcription factor with essential functions throughout development
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DOI：
10.1046/j.1440-169x.2000.00509.x
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发表时间：
2000-06
期刊：
影响因子：
4.6
通讯作者：
Isamu Sugimura;T. Adachi-Yamada;Yoshimi Nishi;Y. Nishida
中科院分区：
文献类型：
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作者：
Isamu Sugimura;T. Adachi-Yamada;Yoshimi Nishi;Y. Nishida
克隆了一个果蝇基因Dwhn (Drosophila whn‐like)，该基因编码一个假定的转录调控因子，其DNA结合域与小鼠的翼螺旋裸体（whn）相似。对次形突变和转基因表达产生的表型分析表明，在复眼、翼静脉和刚毛分化过程中，它在细胞命运决定中起作用。在胚胎发育过程中，Dwhn开始普遍表达，随后是更有限的表达，这与其他果蝇叉头（fkh）家族基因的表达模式形成鲜明对比，后者的局部表达与它们作为局部同源基因的作用密切相关。这种广泛的表达可能对应于强等位基因在胚胎纯合时的多种缺陷，如中枢和周围神经系统的形成缺陷、胚带缩回、头内陷、背闭合等。尽管Dwhn在DNA结合域具有较强的序列保守性，但Dwhn的DNA结合特异性与Whn不同。Dwhn是第一个无脊椎动物中发现的Whn样转录调控因子，它将为研究Whn家族基因的基本功能和进化提供新的思路。
A Drosophila gene, Dwhn (Drosophila whn‐like), encoding a putative transcriptional regulator with a DNA binding domain similar to that of mouse Winged‐helix nude (Whn) was cloned. Analyses of the phenotypes produced by a hypomorphic mutation and transgene expression suggested a role in cell fate decision during the differentiation of the compound eye, wing veins and bristles. During embryonic development, Dwhn expression started ubiquitously followed by more restricted expression in striking contrast to the expression patterns of other Drosophila forkhead (fkh) family genes whose local expression correlate well to their roles as local homeotic genes. This broad expression may correspond to the multiple defects in embryos homozygous for strong alleles, such as defects in the formation of central and peripheral nervous systems, germ band retraction, head involution, and dorsal closure. The DNA binding specificity of Dwhn differed from that of Whn despite the strong sequence conservation in the DNA binding domain. Dwhn is the first invertebrate Whn‐like transcriptional regulator, and should provide insights into the basic functions and evolution of the whn family genes.