Evaluation of in vitro anti-proliferative and immunomodulatory activities of compounds isolated from Curcuma longa
Evaluation of in vitro anti-proliferative and immunomodulatory activities of compounds isolated from Curcuma longa
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DOI：
10.1016/j.fct.2010.04.039
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发表时间：
2010-08-01
影响因子：
4.3
通讯作者：
Lau, Clara B. S.
中科院分区：
文献类型：
--
作者：
Yue, Grace G. L.;Chan, Ben C. L.;Lau, Clara B. S.
姜黄（Curcuma longa， CL）的根茎在亚洲被广泛用作治疗不同疾病的潜在候选药物，包括炎症性疾病和癌症。本研究以人癌细胞HepG2、MCF-7和MDA-MB-231为实验对象，对三种姜黄素和两种姜黄酮化合物的抗增殖活性进行了评价。用人外周血单个核细胞（PBMC）检测了莪术酮（α和芳香族）的免疫调节活性。结果表明，姜黄素（姜黄素、去甲氧基姜黄素和双去甲氧基姜黄素）和α -姜黄酮对肿瘤细胞增殖的抑制作用呈剂量依赖性。这些化合物在癌细胞中的IC50值为11.0 ~ 41.8 μ g/ml。α -姜黄酮诱导MDA-MB-231细胞发生凋亡，annexin-V染色、碘化丙啶染色和DNA片段化实验证实了这一点。在α -姜黄酮处理的细胞中，procaspase -3、-8和-9的显著减少表明caspase级联被激活。α -姜黄酮和芳香型姜黄酮均对PBMC增殖和细胞因子产生有刺激作用。首次发现了-黄酮的抗增殖作用和免疫调节作用。研究结果揭示了丁香粗提取物（含姜黄素和挥发油，包括姜黄酮）作为化学预防剂的潜在用途。(C) 2010 Elsevier Ltd.版权所有。
The rhizome of Curcuma longa (CL) has been commonly used in Asia as a potential candidate for the treatment of different diseases, including inflammatory disorders and cancers. The present study evaluated the anti-proliferative activities of the isolated compounds (three curcuminoids and two turmerones) from CL, using human cancer cell lines HepG2, MCF-7 and MDA-MB-231. The immunomodulatory activities of turmerones (alpha and aromatic) isolated from CL were also examined using human peripheral blood mononuclear cells (PBMC). Our results showed that the curcuminoids (curcumin, demethoxycurcumin and bisdemethoxycurcumin) and alpha-turmerone significantly inhibited proliferation of cancer cells in dose-dependent manner. The IC50 values of these compounds in cancer cells ranged from 11.0 to 41.8 mu g/ml. alpha-Turmerone induced MDA-MB-231 cells to undergo apoptosis, which was confirmed by annexin-V and propidium iodide staining, and DNA fragmentation assay. The caspase cascade was activated as shown by a significant decrease of procaspases-3, -8 and -9 in alpha-turmerone treated cells. Both alpha-turmerone and aromatic-turmerone showed stimulatory effects on PBMC proliferation and cytokine production. The anti-proliferative effect of alpha-turmerone and immunomodulatory activities of alpha-turmerone was shown for the first time. The findings revealed the potential use of CL crude extract (containing curcuminoids and volatile oil including turmerones) as chemopreventive agent. (C) 2010 Elsevier Ltd. All rights reserved.