Maternal prolactin levels during late pregnancy and nurturing behavior of offspring in mice
Maternal prolactin levels during late pregnancy and nurturing behavior of offspring in mice
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小鼠妊娠晚期母体催乳素水平与后代养育行为
DOI：
10.1002/dev.22264
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发表时间：
2022
影响因子：
2.2
通讯作者：
Shimokawa Noriaki
中科院分区：
文献类型：
--
作者：
Masuda Shinnosuke;Ee Oh Kwan;Sairenji Taku James;Sato Seika;Yajima Hiroyuki;Amano Izuki;Koibuchi Noriyuki;Shimokawa Noriaki
阐明养育和忽视行为背后的机制是有意义的，但具有挑战性。最近，我们发现CIN85缺陷小鼠在妊娠后期垂体激素催乳素分泌减少，其幼崽后来表现出抑制的养育行为。为了研究这种现象是否可以在正常小鼠中重现，而不仅仅是CIN85缺陷小鼠，我们研究了在妊娠后期通过溴隐亭给药降低血液催乳素水平的母亲所生后代的养育行为。首先，为了确定何时开始溴隐亭治疗，我们研究了小鼠妊娠晚期血液催乳素水平的详细变化，从而确定了胎盘催乳素激增。此外，催乳素受体在胎儿下丘脑的表达在成人下丘脑相同的程度。溴隐亭治疗降低催乳素的血浆浓度的基础范围在整个妊娠晚期。然而，出乎意料的是，所得到的幼崽表现出成年喂养行为的比例与未接受溴隐亭治疗的小鼠一样高。总之，仅在妊娠晚期消除催乳素分泌不会诱导后代的忽视样行为，这表明CIN85缺陷小鼠似乎涉及除催乳素缺陷外的另一个因素，即CIN85缺陷。
Elucidating the mechanisms underlying nurturing and neglect behaviors is meaningful but challenging. Recently, we found that CIN85‐deficient mice had reduced pituitary hormone prolactin secretion during late pregnancy, and their pups later showed an inhibited nurturing behavior. To examine whether this phenomenon could be reproduced in normal mice and not just CIN85‐deficient mice, we investigated the nurturing behavior of offspring born to mothers whose blood prolactin levels had been reduced by bromocriptine administration during late pregnancy. First, to determine when bromocriptine treatment should be started, we investigated the detailed changes in blood prolactin levels in late pregnancy in mice, resulting in the identification of the prepartum prolactin surge. Furthermore, prolactin receptors in the fetal hypothalamus were expressed to the same extent as in the adult hypothalamus. Treatment with bromocriptine decreased the plasma concentrations of prolactin to the basal range throughout late pregnancy. However, against expectations, the proportion of the resultant pups exhibiting nurturing behaviors as adults was as high as that in the mice without bromocriptine treatment. In conclusion, the elimination of prolactin secretion during late pregnancy alone does not induce neglect‐like behavior in offspring, suggesting that CIN85‐deficient mice appear to involve another factor due to CIN85 deficiency besides prolactin deficiency.