REGULATION OF FATTY-ACID UTILIZATION IN HEART - ROLE OF THE CARNITINE-ACETYL-COA TRANSFERASE AND CARNITINE-ACETYL CARNITINE TRANSLOCASE SYSTEM
REGULATION OF FATTY-ACID UTILIZATION IN HEART - ROLE OF THE CARNITINE-ACETYL-COA TRANSFERASE AND CARNITINE-ACETYL CARNITINE TRANSLOCASE SYSTEM
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DOI：
10.1016/0022-2828(82)90172-9
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发表时间：
1982-01-01
影响因子：
5
通讯作者：
NEELY, JR
中科院分区：
文献类型：
--
作者：
IDELLWENGER, JA;GROTYOHANN, LW;NEELY, JR
本文研究了大鼠心肌线粒体中肉碱-乙酰辅酶A转移酶和肉碱-乙酰肉碱转位酶系统的可逆性。外源性乙酰肉碱可增加线粒体基质乙酰辅酶A水平，降低游离辅酶A水平。总辅酶A和长链辅酶A水平未受影响。当乙酰肉碱的浓度为2.5 mM时，基质乙酰辅酶A的增幅最大。在预先负载乙酰辅酶A的线粒体中加入游离肉碱后，基质乙酰辅酶A迅速下降，游离辅酶A迅速上升，说明基质乙酰辅酶A可以将乙酰基转移到外部乙酰辅酶A，提示肉碱-乙酰-辅酶A转移酶系统能够产生乙酰辅酶A转移酶，而肉碱-乙酰卡尼汀转位酶系统提供了线粒体基质和胞浆空间之间的联系，使胞液脂肪酸激活和线粒体氧化速率偶联。
The reversibility of the carnitine-acetyl-CoA transferase and the carnitine-acetyl carnitine translocase system was studied in mitochondria isolated from rat heart muscle. Incubation of mitochondria with external acetyl-carnitine raised the levels of mitochondrial matrix acetyl-CoA and decreased free CoA. Total CoA and long-chain acyl-CoA levels were not affected. A maximum increase in matrix acetyl-CoA was achieved with an acetyl-carnitine concentration of 2.5 mM. When free carnitine was added to mitochondria preloaded with acetyl-CoA, a rapid decrease in matrix acetyl-CoA and rise in free CoA occurred, illustrating that matrix acetyl-CoA can transfer acetyl units to external acetyl-CoA, suggesting that the carnitine-acetyl-CoA transferase system is capable of producing acetyl-CoA transferase and carnitine-acetyl carnitine translocase system provides the communication between the mitochondrial matrix and cytosolic spaces for coupling rates of cytosolic fatty acid activation with mitochondrial oxidative rates.