Hesperidin Prevents Androgen Deficiency-induced Bone Loss in Male Mice
Hesperidin Prevents Androgen Deficiency-induced Bone Loss in Male Mice
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DOI：
10.1002/ptr.5001
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发表时间：
2014-02-01
影响因子：
7.2
通讯作者：
Uehara, Mariko
中科院分区：
文献类型：
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作者：
Chiba, Hiroshige;Kim, Hyounju;Uehara, Mariko
本研究的目的是检查橙皮苷是否抑制雄激素缺乏的雄性小鼠的骨丢失。7周龄的雄性ddY小鼠进行假手术或睾丸切除术（ORX），并分为五组：假手术组喂食基于AIN-93 G制剂的对照饮食（Sham），其中用玉米油代替大豆油，ORX组喂食对照饮食（ORX），一组喂食含有0.5%橙皮苷的对照饮食（ORX+H），一组喂食含有0.7% -葡糖基橙皮苷（ORX+G）的对照饮食，一组喂食含有0.013%辛伐他汀（ORX+St）的对照饮食。干预4周后，ORX小鼠的精囊重量显着下降，这是不受橙皮苷，β-葡糖基橙皮苷，或辛伐他汀的管理。股骨骨密度显着降低了ORX，和骨丢失抑制橙皮苷，葡糖基橙皮苷或辛伐他汀的管理。组织形态计量学分析显示，ORX组股骨远端松质骨的骨体积和骨小梁厚度显著低于Sham组，破骨细胞数量高于Sham组，并且这些在ORX+H、ORX+G和ORX+St组中正常化。这些结果表明，橙皮苷抑制骨吸收和高脂血症，在ORX小鼠，和预防效果强于我们以前的研究中观察到的卵巢切除小鼠。版权所有（c）2013约翰威利父子有限公司
The purpose of this study was to examine whether hesperidin inhibits bone loss in androgen-deficient male mice. Male ddY mice aged 7 weeks underwent either a sham operation or orchidectomy (ORX) and were divided into five groups: a sham-operated group fed a control diet (Sham) based on AIN-93G formulation with corn oil instead of soy bean oil, an ORX group fed the control diet (ORX), a group fed the control diet containing 0.5% hesperidin (ORX+H), a group fed the control diet containing 0.7% -glucosylhesperidin (ORX+G), and a group fed the control diet containing 0.013% simvastatin (ORX+St). Four weeks after intervention, ORX mice showed a striking decrease in seminal vesicle weight, which was not affected by the administration of hesperidin, -glucosylhesperidin, or simvastatin. Femoral BMD was significantly reduced by ORX, and bone loss was inhibited by the administration of hesperidin, -glucosylhesperidin or simvastatin. Histomorphometric analysis showed that the bone volume and trabecular thickness were significantly lower, and the osteoclast number was higher in the distal femoral cancellous bone in the ORX group than in the Sham group, and these were normalized in the ORX+H, ORX+G and ORX+St groups. These results indicate that hesperidin inhibited bone resorption and hyperlipidemia, in ORX mice, and the preventive effect was stronger than that observed in ovariectomized mice in our previous study. Copyright (c) 2013 John Wiley & Sons, Ltd.