Spatio-temporal performance in an incoherent holography lattice light-sheet microscope (IHLLS)
Spatio-temporal performance in an incoherent holography lattice light-sheet microscope (IHLLS)
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DOI：
10.1364/oe.425069
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发表时间：
2021-07-19
期刊：
影响因子：
3.8
通讯作者：
Alford, Simon
中科院分区：
文献类型：
--
作者：
Potcoava, Mariana;Mann, Christopher;Alford, Simon
我们提出了一种用于晶格光片（ihls）系统的非相干全息检测技术，用于3D成像，无需移动样品台或检测显微镜物镜，与原始的LLS方案相比，提供了固有的仪器简单性和高精度。该方法基于改进的双透镜菲涅耳非相干相关全息技术来产生复杂的全息图，并提供重建全息图所需的焦距。我们报告了这样一种ihls显微镜，包括传感器性能的表征，并在生物测试样品和定量相位成像中展示了珠子和神经元结构的显着对比度改进。与LLS技术相比，IHLLS具有相似或更好的横向性能。此外，ihls允许从更少的z-galvo位移中进行体积重建，从而促进更快的体积采集。(C) 2021年美国光学学会根据OSA开放获取出版协议的条款
We propose an Incoherent holography detection technique for lattice light-sheet (IHLLS) systems for 3D imaging without moving either the sample stage or the detection microscope objective, providing intrinsic instrumental simplicity and high accuracy when compared to the original LLS schemes. The approach is based on a modified dual-lens Fresnel Incoherent Correlation Holography technique to produce a complex hologram and to provide the focal distance needed for the hologram reconstruction. We report such an IHLLS microscope, including characterization of the sensor performance, and demonstrate a significant contrast improvement on beads and neuronal structures within a biological test sample as well as quantitative phase imaging. The IHLLS has similar or better transverse performances when compared to the LLS technique. In addition, the IHLLS allows for volume reconstruction from fewer z-galvo displacements, thus facilitating faster volume acquisition. (C) 2021 Optical Society of America under the terms of the OSA Open Access Publishing Agreement