Location of an inducible nitric oxide synthase mRNA in the normal kidney.
Location of an inducible nitric oxide synthase mRNA in the normal kidney.
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DOI：
10.1038/ki.1994.135
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发表时间：
1994-04
影响因子：
19.6
通讯作者：
J. Morrissey;R. McCracken;H. Kaneto;M. Vehaskari;D. Montani;S. Klahr
中科院分区：
文献类型：
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作者：
J. Morrissey;R. McCracken;H. Kaneto;M. Vehaskari;D. Montani;S. Klahr
诱导型一氧化氮合酶mRNA在正常肾脏中的定位。诱导型一氧化氮合酶（iNOS）mRNA主要存在于正常大鼠肾脏的外髓质。mRNA的鉴定基于用于PCR扩增的寡核苷酸引物的特异性、PCR-Southern印迹分析和克隆的PCR产物的核酸序列。除了外髓质，从正常大鼠肾脏制备的肾小球中含有大量的iNOS mRNA。这些结果表明，可能有紧张性的影响，在正常大鼠肾脏的外髓质导致的“稳态”存在的一种诱导型一氧化氮合酶mRNA。皮质小管和内髓质被发现含有可检测到的，但数量较少的iNOS mRNA。将外髓质显微解剖为近端直小管（PST）、髓质粗升支（MTAL）、髓质集合管（MCD）和直血管束（VRB）。在正常大鼠肾组织中，iNOSmRNA主要分布于MTAL，MCD和VRB中也有少量表达。给动物注射脂多糖（LPS）并在24小时后处死。用LPS处理引起肝脏或从腹膜分离的巨噬细胞中iNOS mRNA的量增加至少20倍。内毒素处理导致肾小球和内髓质中iNOS mRNA含量增加10倍以上。外髓的iNOSmRNA水平增加了2 - 3倍，由于LPS治疗。
Location of an inducible nitric oxide synthase mRNA in the normal kidney. An inducible nitric oxide synthase (iNOS) mRNA was found primarily in the outer medulla of normal rat kidney. Identification of the mRNA was based upon the specificity of the oligonucleotide primers used for PCR amplification, PCR-Southern blot analysis and the nucleic acid sequence of the cloned PCR product. In addition to the outer medulla, glomeruli prepared from normal rat kidney contained significant amounts of an iNOS mRNA. These results suggest that there may be tonic influences in the outer medulla of the normal rat kidney resulting in the “steady-state” presence of an iNOS mRNA. Cortical tubules and the inner medulla were found to contain detectable but lesser amounts of the iNOS mRNA. The outer medulla was microdis-sected into proximal straight tubule (PST), medullary thick ascending limb (MTAL), medullary collecting duct (MCD) and vasa recta bundle (VRB). The iNOS mRNA was found primarily in the MTAL with minor amounts in the MCD and VRB of normal rat kidney. Animals were injected with lipopolysaccharide (LPS) and sacrificed 24 hours later. Treatment with LPS caused at least a 20-fold increase in the amount of iNOS mRNA in the liver or in macrophages isolated from the peritoneum. Endotoxin treatment led to over a 10-fold increase in iNOS mRNA content in glomeruli and the inner medulla. The iNOS mRNA level of the outer medulla was increased two- to threefold due to LPS treatment.