Effects of extracts from Italian medicinal plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus
Effects of extracts from Italian medicinal plants on planktonic growth, biofilm formation and adherence of methicillin-resistant Staphylococcus aureus
复制标题
DOI：
10.1016/j.jep.2008.05.005
复制
发表时间：
2008-08-13
影响因子：
5.4
通讯作者：
Bennett, Bradley C.
中科院分区：
文献类型：
--
作者：
Quave, Cassandra L.;Plano, Lisa R. W.;Bennett, Bradley C.
研究目的：意大利南部三分之一的植物疗法用于治疗皮肤和软组织感染（SSTI）。金黄色葡萄球菌（Staphylococcus aureus）是SM的常见病因，由于耐药而引起越来越多的关注。许多植物含有抗菌物质，为SSTI提供有效的治疗方法。我们的目的是研究不同民族植物使用群的植物对耐甲氧西林金黄色葡萄球菌（MRSA）生长和生物膜的抑制作用。材料和方法：对三组进行评估：治疗SSTI的植物疗法，不涉及皮肤的植物疗法。以及没有民族医学用途的植物。我们筛选了168个提取物，代表104个植物物种，对MRSA的活性（ATCC 33593）。我们采用肉汤稀释法测定生长18 h后的MIC，使用光密度（OD(600nm)）读数。通过培养生物膜40 h，然后用结晶紫进行固定和染色，评价生物膜的抗生物膜作用。洗涤后，加入10%吐温80，测定外径（570nm）。结果：10种植物提取物均表现出IC(50)；
Aim of study: One-third of botanical remedies from southern Italy are used to treat skin and soft tissue infection (SSTI). Staphylococcus aureus, a common cause of SM, has generated increasing concern due to drug resistance. Many plants possess antimicrobial agents and provide effective remedies for SSTI. Our aim was to investigate plants from different ethnobotanical usage groups for inhibition of growth and biofilms in methicillin-resistant Staphylococcus aureus (MRSA).Materials and methods: Three groups were assessed: plant remedies for SSTI, plant remedies not involving the skin. and plants with no ethnomedical application. We screened 168 extracts, representing 104 botanical species, for activity against MRSA (ATCC 33593). We employed broth dilution methods to determine the MIC after 18 h growth using an optical density (OD(600nm)) reading. Anti-biofilm effects were assessed by growing biofilms for 40 h, then fixing and staining with crystal violet. After washing, 10% Tween 80 was added and OD(570nm) readings were taken.Results: Extracts from 10 plants exhibited an IC(50)