An Open Model of Actin Dendritic Nucleation
An Open Model of Actin Dendritic Nucleation
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DOI：
10.1016/j.bpj.2009.01.037
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发表时间：
2009-05-06
影响因子：
3.4
通讯作者：
Loew, Leslie M.
中科院分区：
文献类型：
--
作者：
Ditlev, Jonathon A.;Vacanti, Nathaniel M.;Loew, Leslie M.
肌动蛋白组装动力学的定量实验数据的可用性使得许多数学模型的构建专注于解释这个复杂系统的特定行为。然而，这些特别的模型通常不能被肌动蛋白生物学家的大社区重用或访问。在这项工作中，我们提出了一个全面的模型，该模型集成并统一了许多关于肌动蛋白动力学树突成核机制组成部分的体外数据。基于该模型的隔间和三维空间版本，已经进行了300多次模拟。几个关键的发现被强调，包括解释肌动蛋白组装和拆卸之间的尖锐边界在迁移细胞的足部。因为这个模型和仿真结果是“开源的”，也就是说它可以通过Virtual Cell数据库（http://vcell.org）公开获得和编辑，所以它可以被访问、分析、修改和扩展。
The availability of quantitative experimental data on the kinetics of actin assembly has enabled the construction of many mathematical models focused on explaining specific behaviors of this complex system. However these ad hoc models are generally not reusable or accessible by the large community of actin biologists. In this work, we present a comprehensive model that integrates and unifies much of the in vitro data on the components of the dendritic nucleation mechanism for actin dynamics. More than 300 simulations have been run based on compartmental and three-dimensional spatial versions of this model. Several key findings are highlighted, including an explanation for the sharp boundary between actin assembly and disassembly in the lannellipodia of migrating cells. Because this model, with the simulation results, is "open source", in the sense that it is publicly available and editable through the Virtual Cell database (http://vcell.org), it can be accessed, analyzed, modified, and extended.