Imaging of germinal center selection events during affinity maturation
Imaging of germinal center selection events during affinity maturation
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DOI：
10.1126/science.1136736
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发表时间：
2007-01-26
期刊：
影响因子：
56.9
通讯作者：
Cyster, Jason G.
中科院分区：
文献类型：
--
作者：
Allen, Christopher D. C.;Okada, Takaharu;Cyster, Jason G.
生发中心（GC）是产生和选择携带高亲和力抗体的B细胞的重要场所，但GC细胞迁移和相互作用动力学尚未直接观察到。使用双光子显微镜的小鼠淋巴结，我们发现，GC B细胞是高度能动的，并延长长的细胞突起。尽管这些B细胞仅在展示抗原的亮区停留数小时，但它们在GC暗区和亮区之间过渡并在两个区域中分裂。GC B细胞与GC T细胞尽管经常相遇，但很少形成稳定的接触，并且观察到T细胞携带死亡的B细胞泡。在这些观察的基础上，我们提出了一个模型，在该模型中，T细胞的帮助竞争在GC B细胞的选择中起着比以前更主导的作用。
The germinal center ( GC) is an important site for the generation and selection of B cells bearing high-affinity antibodies, yet GC cell migration and interaction dynamics have not been directly observed. Using two-photon microscopy of mouse lymph nodes, we revealed that GC B cells are highly motile and extend long cell processes. They transited between GC dark and light zones and divided in both regions, although these B cells resided for only several hours in the light zone where antigen is displayed. GC B cells formed few stable contacts with GC T cells despite frequent encounters, and T cells were seen to carry dead B cell blebs. On the basis of these observations, we propose a model in which competition for T cell help plays a more dominant role in the selection of GC B cells than previously appreciated.