KNOX homeobox genes are sufficient in maintaining cultured cells in an undifferentiated state in rice
KNOX homeobox genes are sufficient in maintaining cultured cells in an undifferentiated state in rice
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DOI：
10.1002/gene.1069
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发表时间：
2001-08-01
期刊：
影响因子：
1.5
通讯作者：
Kurata, N
中科院分区：
文献类型：
--
作者：
Ito, Y;Eiguchi, M;Kurata, N
我们培育了组成性表达水稻KNOX家族1类同源盒基因OSH1、OSH16、OSH15和OSH71的转基因水稻愈伤组织，发现这些基因的组成性和异位表达抑制了转化的愈伤组织的正常再生，这些愈伤组织在茎再生阶段表现出持续生长。转基因打电话!转移到再生培养基上开始出现绿斑，这是再生的标志，但大多数转化体在给定阶段继续繁殖绿斑。在愈伤组织正常的茎再生过程中，内源OSH1的表达在愈伤组织推定的茎再生区域受到限制，而在其他区域未观察到。这种受限的表达模式对于再生芽的进一步分化是必需的。因此，我们目前的研究结果支持KNOX家族1类同源盒基因在细胞未确定的分生组织状态的形成和维持中发挥作用的提议功能。(C) 2001 Wiley-Liss, Inc。
We produced transgenic rice calli, which constitutively express each of four KNOX family class 1 homeobox genes of rice, OSH1, OSH16, OSH15, and OSH71, and found that constitutive and ectopic expression of such genes inhibits normal regeneration from transformed calli, which showed continuous growth around their shoot-regenerating stages. Transgenic call! transferred onto regeneration medium began to display green spots, a sign of regeneration, but most of the transformants continued to propagate green spots at given stages. In the normal shoot-regeneration process of calli, expression of endogenous OSH1 was restricted in presumptive shoot-regenerating regions of calli and not observed in other areas. This restricted expression pattern should be required for further differentiation of the regenerating shoots. Thus our present results support the proposed function that KNOX family class 1 homeobox genes play a role in the formation and maintenance of the undetermined meristematic state of cells. (C) 2001 Wiley-Liss, Inc.