Effect of sulfidopeptide leukotrienes D4 and E4 on ileal ion transport in vitro in the rat and rabbit.
Effect of sulfidopeptide leukotrienes D4 and E4 on ileal ion transport in vitro in the rat and rabbit.
复制标题
硫肽白三烯 D4 和 E4 对大鼠和兔体外回肠离子转运的影响。
DOI：
10.1152/ajpgi.1988.255.2.g175
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发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Fondacaro,JD
中科院分区：
文献类型：
--
作者：
Smith,PL;Montzka,DP;McCafferty,GP;Wasserman,MA;Fondacaro,JD
白三烯D4和E4（LTD 4和LTE 4）对电解质转运的影响进行了研究，采用剥离的大鼠和兔回肠段安装在Ussing室。在血清中加入LTD 4或LTE 4，但在粘膜浴液中不加入LTD 4或LTE 4，可引起短路电流（Isc）短暂增加，在大鼠和家兔中分别在10（-5）M和10（-8）M时观察到最大反应，仅在大鼠中引起跨上皮电导（Gt）持续降低。在大鼠中，氯替代，减少洗澡溶液[Ca 2 +]至1 μ M或预处理1 μ M吲哚美辛或甲异柳胺酸抑制LTD 4或LTE 4诱导的Isc的变化，而对Gt.LTD4（10 μ M）的减少没有影响短暂增加净氯分泌，并产生了持续的单向和净钠运输和粘膜-serumCl通量在大鼠回肠减少。单向钠通量的减少主要是由潜在的独立钠通量的变化。这些结果表明，增加在大鼠和兔的Isc是由花生四烯酸代谢产物介导的，而减少Gt和净钠吸收在大鼠回肠介导的环加氧酶非依赖性途径。
Effects of leukotrienes D4 and E4 (LTD4 and LTE4) on electrolyte transport were examined, employing stripped segments of rat and rabbit ileum mounted in Ussing chambers. Addition of LTD4 or LTE4 to the serosal but not the mucosal bathing solution elicited a transient increase in short-circuit current (Isc) with maximal responses seen at 10(-5) M and 10(-8) M in rat and rabbit respectively and a sustained decrease in transepithelial conductance (Gt) in the rat only. In the rat, Cl replacement, reduction of bathing solution [Ca2+] to 1 microM or pretreatment with 1 microM indomethacin or meclofenamic acid inhibited the LTD4- or LTE4-induced Isc changes with no effect on the decrease in Gt. LTD4 (10 microM) transiently increased net Cl secretion and produced a sustained decrease in both unidirectional and net Na transport and mucosal-to-serosal Cl flux in rat ileum. The decrease in unidirectional Na fluxes is accounted for predominantly by a change in the potential independent flux of Na. These results suggest that the increase in Isc in both rat and rabbit is mediated by arachidonic acid metabolites, whereas the decrease in Gt and net Na absorption in rat ileum is mediated by a cyclooxygenase-independent pathway.