A novel amperometric biosensor based on gold nanoparticles anchored on reduced graphene oxide for sensitive detection of L-lactate tumor biomarker
A novel amperometric biosensor based on gold nanoparticles anchored on reduced graphene oxide for sensitive detection of L-lactate tumor biomarker
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DOI：
10.1016/j.bios.2015.03.012
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发表时间：
2015-07-15
影响因子：
12.6
通讯作者：
Bala, Camelia
中科院分区：
文献类型：
--
作者：
Azzouzi, Sawsen;Rotariu, Lucian;Bala, Camelia
在这项工作中，一种新的安培型生物传感器的基础上锚定的还原氧化石墨烯（RGO-AuNPs）和L-乳酸脱氢酶（LDH）的L-乳酸的传感。首先，测试RGO-AuNP修饰的丝网印刷电极用于NADH检测，显示出宽的动态范围和低的检测限。接下来，通过将酶和RGO-AuNP两者并入源自四甲氧基硅烷和甲基三甲氧基硅烷的溶胶凝胶基质中来构建生物传感器。对酶的负载量、工作pH和辅酶浓度进行了优化。该生物传感器对L-乳酸在10 μ M-5 mM范围内呈线性响应，比灵敏度为154 μ A/mM cm（2），检出限为0.13 μ M。这是伴随着良好的再现性和操作稳定性。人工血清试验证明L.-实际上可以测定乳酸盐而不受通常干扰化合物如尿酸盐、扑热息痛和L-抗坏血酸盐的干扰。因此，我们的基于LDH/RGO-AuNP/SPCE的生物传感器作为电化学装置用于检测作为可行的早期癌症生物标志物的L-乳酸。(C)2015爱思唯尔B. V.保留所有权利。
In this work, a novel amperometric biosensor based on gold nanoparticles anchored on reduced graphene oxide (RGO-AuNPs) and L-lactate dehydrogenase (LDH) was developed for the sensing of L-lactate. Firstly, the RGO-AuNPs modified screen printed electrodes were tested for NADH detection showing a wide dynamic range and a low detection limit. Next, the biosensor was constructed by incorporating both enzyme and RGO-AuNPs in a sol gel matrix derived from tetrametoxysilane and methyltrimetoxysilane. The enzyme loading, working pH, and coenzyme concentration were optimized. The biosensor linearly responded to L-lactate in the range of 10 mu M-5 mM and showed a good specific sensitivity of 154 mu A/mM cm(2) with a detection limit of 0.13 mu M. This was accompanied by good reproducibility and operational stability. Tests on artificial serum proved that L.-lactate can be determined practically without interferences from commonly interfering compounds such as urate, paracetamol and L-ascorbate. Our LDH/RGO-AuNPs/SPCE based biosensor thus performs as electrochemical device for the detection of L-lactate as a viable early cancer bio-marker. (C) 2015 Elsevier B.V. All rights reserved.