Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations.
Site-specific labeling of Saccharomyces cerevisiae ribosomes for single-molecule manipulations.
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DOI：
10.1093/nar/gkq390
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发表时间：
2010-07
影响因子：
14.9
通讯作者：
Puglisi JD
中科院分区：
文献类型：
--
作者：
Petrov A;Puglisi JD
Site-specific labeling of Escherichia coli ribosomes has allowed application of single-molecule fluorescence spectroscopy and force methods to probe the mechanism of translation. To apply these approaches to eukaryotic translation, eukaryotic ribosomes must be specifically labeled with fluorescent labels and molecular handles. Here, we describe preparation and labeling of the small and large yeast ribosomal subunits. Phylogenetically variable hairpin loops in ribosomal RNA are mutated to allow hybridization of oligonucleotides to mutant ribosomes. We demonstrate specific labeling of the ribosomal subunits, and their use in single-molecule fluorescence and force experiments.
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影响因子：
3.4
作者：
Aitken, Colin Echeverria;Marshall, R. Andrew;Puglisi, Joseph D.
通讯作者：
Puglisi, Joseph D.
影响因子：
16
作者：
Marshall, R. Andrew;Aitken, Colin Echeverria;Puglisi, Joseph D.
通讯作者：
Puglisi, Joseph D.
DOI：
10.1073/pnas.0805299105
发表时间：
2008-10-07
影响因子：
11.1
作者：
Marshall, R. Andrew;Dorywalska, Magdalena;Puglisi, Joseph D.
通讯作者：
Puglisi, Joseph D.
DOI：
10.1073/pnas.0908077106
发表时间：
2009-09-15
影响因子：
11.1
作者：
Fei, Jingyi;Bronson, Jonathan E.;Gonzalez, Ruben L., Jr.
通讯作者：
Gonzalez, Ruben L., Jr.
影响因子：
5.3
作者：
Rakauskaite, Rasa;Dinman, Jonathan D.
通讯作者：
Dinman, Jonathan D.