Novel Epitopic Region of Glucosyltransferase B from Streptococcus mutans
Novel Epitopic Region of Glucosyltransferase B from Streptococcus mutans
复制标题
DOI：
10.1128/cvi.05041-11
复制
发表时间：
2011-09-01
影响因子：
--
通讯作者：
Fujiwara, Taku
中科院分区：
文献类型：
--
作者：
Hoshino, Tomonori;Kondo, Yoshio;Fujiwara, Taku
变形链球菌葡糖基转移酶B（Gtf B）的催化区（CAT）和葡聚糖结合区（GBD）已被用作防龋组分疫苗的靶抗原。这些区域被采用作为主要靶标，因为它们理论上包括与酶功能相关的表位。然而，其抗原性尚未得到充分评价。虽然有许多关于使用这些成分成功接种疫苗的报道，但该原理尚未投入实际使用。由于这些原因，我们开始怀疑表位在疫苗生产中的有效性，并通过使用计算机分析结合体外和体内实验重新评估GtfB的抗原区域。结果表明，CA。GtfB的N末端的360个氨基酸的可变区（VR）比CAT和GBD更具有反应性。这个区域是S。变形链球菌和/或GtfB特异性的，在其他链球菌Gtf中不保守的，并且功能未知。使用腺病毒载体携带的DNA疫苗的免疫证实，VR是一个表位，显示龋齿疫苗的发展前景。
In the development of a component vaccine against caries, the catalytic region (CAT) and glucan-binding domain (GBD) of glucosyltransferase B (GtfB) from Streptococcus mutans have been employed as target antigens. These regions were adopted as primary targets because they theoretically include epitopes associated with enzyme function. However, their antigenicities have not been fully evaluated. Although there are many reports about successful vaccination using these components, the principle has not yet been put to practical use. For these reasons, we came to doubt the effectiveness of the epitopes in vaccine production and reevaluated the antigenic region of GtfB by using in silico analyses combined with in vitro and in vivo experiments. The results suggested that the ca. 360-amino-acid variable region (VR) in the N terminus of GtfB is more reactive than CAT and GBD. This region is S. mutans and/or GtfB specific, nonconserved among other streptococcal Gtfs, and of unknown function. Immunization using an adenovirus vector-borne DNA vaccine confirmed that VR is an epitope that shows promise for the development of a caries vaccine.